il-1β对人皮肤瘢痕成纤维细胞增殖及胶原蛋白代谢的影响

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1、ཚ৏⨶ᐕབྷᆖ⺅༛⹄ウ⭏ᆖս䇪᮷IL-1ȕሯӰⳤ㛚ⱘⰋᡆ㓚㔪㓼㜔໔⇌਀㜬৕㴁ⲳԙ䉘Ⲻᖧଃ᪎㾷໎⭏ᙗⱒⰅᱟӪփⳞ㛔ⵏⳞቲਇࡠࡋՔਾᕅ䎧Ⲵа㌫ࡇⵏⳞቲ໎⭏DŽ൘ࡋՔ؞༽䗷〻ѝˈᡀ㓔㔤㓶㜎䎧ࡠ㠣ޣ䟽㾱Ⲵ֌⭘ˈҶ䀓઼᧗ࡦᡀ㓔㔤㓶㜎Ⲵ⭏⢙ᆖ㹼Ѫᱟ׳䘋Քਓ᜸ਸ઼亴䱢ⱒⰅᖒᡀⲴส⹰઼ޣ䭞DŽ䘁ᒤᶕˈ䲿⵰⧠ԓ㓶㜎⭏⢙ᆖ઼࠶ᆀ⭏⢙ᆖ൘ⱒⰅ亶ฏⲴ␡ޕ⹄ウ઼䗵䙏ਁኅˈሩᡀ㓔㔤㓶㜎ǃ㓶㜎ཆส䍘઼㓶㜎ഐᆀй㘵ѻ䰤Ⲵ⴨ӂ֌⭘ᴹҶ䘋а↕Ⲵ䇔䇶DŽᡀ㓔㔤㓶㜎ਟԕ࠶⋼㓶㜎ཆส䍘ˈ㘼ᡀ㓔㔤㓶㜎Ⲵབྷ䟿໎⇆ǃ㓶㜎ཆส䍘ѝ㜦৏ਸᡀо䱽䀓ǃ䜘࠶㓶㜎ഐᆀⲴབྷ

2、䟿ӗ⭏ԕ৺й㘵ѻ䰤Ⲵᇶ࠷ޣ㌫ᶴᡀҶ໎⭏ᙗⱒⰅᖒᡀⲴ⭏⢙ᆖส⹰DŽ⹄ウਁ⧠ˈ൘ࡋՔࡍᵏˈ㺘Ⳟ㓶㜎઼ⵏⳞ㓶㜎ሶ࠶⋼ⲭӻ㍐-1ȕ(Interleukin-1ȕˈIL-1ȕ)ˈфIL-1ȕާᴹ׳䘋ᡀ㓔㔤㓶㜎ǃ㹰㇑޵Ⳟ㓶㜎໎⇆઼㓶㜎ཆส䍘⊹〟֌⭘DŽᵜ᮷ᇎ傼൘㓶㜎≤ᒣˈᒦ㔃ਸ⭏⢙ᆖⲴᯩ⌅઼᡻⇥ˈ⹄ウҶӪⳞ㛔ᡀ㓔㔤㓶㜎(humanskinfibroblasts,HFB)઼ⱒⰅᡀ㓔㔤㓶㜎(hypertrophicscarfibroblasts,HSF)൘⿫փ⣦ᘱлˈн਼⎃ᓖཆⓀᙗⲴIL-1ȕࡪ◰ਾˈަ໎⇆ᛵߥ઼㓶㜎ཆส䍘ѝ

3、㜦৏㳻ⲭԓ䉒Ⲵ᭩ਈˈᴹࣙҾ䘋а↕Ҷ䀓ⱒⰅᖒᡀⲴ⭏⢙ᆖᵪࡦԕ৺Ѫ໎⭏ᙗⱒⰅⲴ⋫⯇ᯩ⌅৺䇴ՠᨀ׋䟽㾱৲㘳DŽᵜ᮷Ⲵѫ㾱ᐕ֌৺㔃䇪ྲл˖(1)䟷⭘㓴㓷ඇ䍤໱⌅ᨀਆᒦษޫHSFˈ䟷⭘㜦৏䞦⎸ॆ⌅ᨀਆᒦษޫHFBˈHSFоHFB൘ᖒᘱкᰐ᰾ᱮᐞᔲˈ൷੸䮯ờᖒ˗ᵜᇎ傼൷⭘ㅜ3~54ԓ⭏䮯⣦ᘱ㢟ྭф༴Ҿሩᮠ໎⇆ᵏⲴ㓶㜎ˈԕ⇿ᆄ1.5×10њ㓶㜎Ⲵᇶᓖ᧕Iཚ৏⨶ᐕབྷᆖ⺅༛⹄ウ⭏ᆖս䇪᮷3⿽Ҿ6ᆄษޫᶯᡆ10њ㓶㜎Ⲵᇶᓖ᧕⿽Ҿ96ᆄษޫᶯˈ24hਾˈ࠶࡛⭘2.5ǃ5ǃ10о20ng/mL⎃ᓖⲴIL-1ȕษޫ㓶㜎24hˈԕ0n

4、g/mL⎃ᓖⲴIL-1ȕษޫ㓶㜎24hѪሩ➗㓴˗Ự⍻㓶㜎໎⇆ԕ৺㓶㜎ཆส䍘ѝ㜦৏㳻ⲭԓ䉒Ⲵ⴨ޣᤷḷDŽ(2)䟷⭘MTT∄㢢⌅⍻ᇊ਴㓴㓶㜎Ⲵ໎⇆ᛵߥˈ⍱ᔿ㓶㜎࠶᷀⌅⍻ᇊ਴㓴㓶㜎Ⲵ㓶㜎ઘᵏˈᒦ࠶᷀਴㓴ᡀ㓔㔤㓶㜎Sᵏᡰঐ∄DŽк䘠є⿽Ự⍻ᯩ⌅൷ᗇࠪ˖IL-1ȕਟԕ׳䘋HFB໎⇆ˈᣁࡦHSF໎⇆ˈӾ㘼ᣁࡦⱒⰅⲴᖒᡀ˗ᒦф䲿⵰ษޫ⏢ѝIL-1ȕ⎃ᓖⲴॷ儈ˈަ׳䘋HFBⲴ໎⇆֌⭘䎺᰾ᱮˈᣁࡦHSFⲴ໎⇆ҏ䎺᰾ᱮDŽ(3)䟷⭘ELISA⌅Ự⍻਴㓴ᡀ㓔㔤㓶㜎к␵⏢ѝIර㜦৏㳻ⲭ઼ɒර㜦৏㳻ⲭⲴ⎃ᓖDŽк䘠Ự⍻ᯩ⌅ᗇࠪ˖IL-1ȕ

5、ਟԕ׳䘋HFBਸᡀᒦ࠶⋼Iǃɒර㜦৏㳻ⲭˈᣁࡦHSFਸᡀ࠶⋼Iǃɒර㜦৏㳻ⲭˈӾ㘼ᣁࡦⱒⰅਁ⭏˗ᒦф䲿⵰ษޫ⏢ѝIL-1ȕ⎃ᓖॷ儈ˈަ׳䘋HFB㜦৏㳻ⲭԓ䉒Ⲵ֌⭘䎺᰾ᱮˈᣁࡦHSF㜦৏㳻ⲭԓ䉒Ⲵ֌⭘ҏ䎺᰾ᱮDŽIL-1ȕࡪ◰㜭ཏᖡ૽ᡀ㓔㔤㓶㜎Iǃɒර㜦৏㳻ⲭⲴਸᡀо࠶⋼ˈфоIL-1ȕⲴ⎃ᓖᴹޣˈᵏᵋ䈕㔃䇪㜭Ѫ⴨ޣ⯮⯵Ѥᒺ⭘㦟ࡲ䟿᧗ࡦᨀ׋⨶䇪׍ᦞDŽީ䭤䈃φ໎⭏ᙗⱒⰅˈᡀ㓔㔤㓶㜎ˈIL-1ȕˈ໎⇆ˈ㜦৏ԓ䉒IIཚ৏⨶ᐕབྷᆖ⺅༛⹄ウ⭏ᆖս䇪᮷EFFECTSOFIL-1ȕONPROLIFERATIONANDCOL

6、LAGENEXPRESSIONOFHYPERTROPHICSCARFIBROBLASTSABSTRACTHypertrophicscarisaseriesofhyperplasiaofdermisafterdeepdermalwounds.Fibroblastsplayacrucialroleintheprocessofwoundrepair.Tounderstandandcontrolthebiologicalbehaviouroffibroblastsisthefoundationandkeyofpr

7、omotingwoundhealingandpreventingtheformationofscar.Inrecentyears,astherapiddevelopmentandresearchofmoderncellbiologyandmolecularbiologyinthefieldofscar,wehaveafurtherunderstandingoftheinteractionsbetweenfibroblasts,extracellularmatrixandcytokine.Weacquire

8、thatfibroblastscansecreteofextracellularmatrix.However,theproliferationoffibroblast,synthesisanddegradationofcollageninextracellularmatrix,thegreatproductionofsectionalcytokinesandthecloserelationshipbetweenthethree