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时间:2018-09-15
《祛风通络方对阿霉素肾病大鼠肾小球基底膜hspg蛋白及mrna表达的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据刚”大学学%医学E㈣di2010;JSichuanUnivedSciEdi)4l(2).212.217(M⋯⋯⋯⋯祛风通络方对阿霉素肾病大鼠肾小球基底膜HSPG蛋白及mRNA表达的影响*孙万森,任艳芸,赵艳龙,马巧亚西安交通大学医学院第二附属医院中西医结合科(西安710004)【摘要】目的观察阿霉素肾病大鼠肾小球基底膜硫酸肝索蛋白多糖(HSPG)蛋白及其mRNA表达及祛风通络方对其表达的影响。方法尾静脉注射阿霉素建立阿霉素肾病大鼠模型,并随机分为A(空白对照,,l=32)、B(模型,n=18)、C(祛风通络方。靠=18)、
2、D(祛风通络方+激素,珂=18)、E(激素,咒=18)、F(苯那普利,行=18)组.应用间接免疫荧光染色、RT-PCR分别检测各组大鼠肾小球基底膜HSPG的荧光分布和HSPGmRNA表达的变化。结果造模成功后透射电镜示B组肾小球足突呈部分或弥漫性融合。干预结束后与B组相比,C、E组HSPG的荧光分布呈连续性或斑片状,平均荧光强度明显增强(P3、析显示24h尿蛋白定量与HSPG蛋白表达呈明显负相关(r------0.7353,P<0.001)。结论肾小球基底膜HSPG的表达异常和分布改变是阿霉索肾病大鼠蛋白尿发生发展的重要机制,祛风通络方可能通过改变HSPG的表达和分布而达到治疗蛋白尿的目的。【关键词】阿霉索肾病蛋白尿硫酸肝素蛋白多糖祛风通络方【中图分类号】R285.5EffectsofQufengtongluoRecipeonExpressionsofHSPGmRNAandProteininAdriamycin-inducedNephropathyRatsSUNWan-4、sen。RENYan-yun,ZHA0‰行一long,MAQiao-ya.DepartmentofTraditionalandWesternMedicine。theSecondAffiliatedHospital。MedicalSchoolofXi’anJiaotongUniversity.Xi’an710004,China[Abstract]ObjectiveToinvestigatetheexpressionofHSPGinglomerularbasemembraneofadriamycin-inducednephropath5、y(AN)rats,andtheeffectofQufengtongluorecipeonHSPGmRNAexpressionandproteinuriainANrats.MethodsOnehundredfortyratswereusedinthisstudy,including32ratsinnormalcontrolgroup.ANwasinducedintheleftratsbyasingletailintravenousinjectionofadriamycin.Threeweekslater,90ANratswerer6、andomlydividedintofivegroups:thenephropathygroup(B,n。18),theQufenggroup(C,疗一18),Qufengandprednisonegroup(D,n=18)。prednisonegroup(E,竹=18)andbenazeprigroup(F,n=18).TheratsinthesefivegroupsweretreatedwithdifferentcombinationofQufengrecipeandprednisone.Ineachgroup,renalti7、ssuesampleswerecollectedatweek3and7.Thedistribution,expressionofHSPGwasexaminedbyindirectimmunofluorescence,andsemi—quantityRT-PCRrespeetivelyResults①InANrats,thediffusefusionandeffacementOffOOtprocesseswereobservedwhenmodelestablished.②Comparedwithnephropathygroup,th8、eaveragefluorescenceintensityofHSPGdramaticallyincreasedinQufenggroupandprednisonegroup(P
3、析显示24h尿蛋白定量与HSPG蛋白表达呈明显负相关(r------0.7353,P<0.001)。结论肾小球基底膜HSPG的表达异常和分布改变是阿霉索肾病大鼠蛋白尿发生发展的重要机制,祛风通络方可能通过改变HSPG的表达和分布而达到治疗蛋白尿的目的。【关键词】阿霉索肾病蛋白尿硫酸肝素蛋白多糖祛风通络方【中图分类号】R285.5EffectsofQufengtongluoRecipeonExpressionsofHSPGmRNAandProteininAdriamycin-inducedNephropathyRatsSUNWan-
4、sen。RENYan-yun,ZHA0‰行一long,MAQiao-ya.DepartmentofTraditionalandWesternMedicine。theSecondAffiliatedHospital。MedicalSchoolofXi’anJiaotongUniversity.Xi’an710004,China[Abstract]ObjectiveToinvestigatetheexpressionofHSPGinglomerularbasemembraneofadriamycin-inducednephropath
5、y(AN)rats,andtheeffectofQufengtongluorecipeonHSPGmRNAexpressionandproteinuriainANrats.MethodsOnehundredfortyratswereusedinthisstudy,including32ratsinnormalcontrolgroup.ANwasinducedintheleftratsbyasingletailintravenousinjectionofadriamycin.Threeweekslater,90ANratswerer
6、andomlydividedintofivegroups:thenephropathygroup(B,n。18),theQufenggroup(C,疗一18),Qufengandprednisonegroup(D,n=18)。prednisonegroup(E,竹=18)andbenazeprigroup(F,n=18).TheratsinthesefivegroupsweretreatedwithdifferentcombinationofQufengrecipeandprednisone.Ineachgroup,renalti
7、ssuesampleswerecollectedatweek3and7.Thedistribution,expressionofHSPGwasexaminedbyindirectimmunofluorescence,andsemi—quantityRT-PCRrespeetivelyResults①InANrats,thediffusefusionandeffacementOffOOtprocesseswereobservedwhenmodelestablished.②Comparedwithnephropathygroup,th
8、eaveragefluorescenceintensityofHSPGdramaticallyincreasedinQufenggroupandprednisonegroup(P
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