脂肪干细胞与软骨细胞的共培养

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1、万方数据中国组织T程研究与临床康复笫f3誊第24嬲2009—06—11出版JournalofClinicalRehabilitativeTissueEngineeringResearchJune11，2009V01．13No．24脂肪干细胞与软骨细胞的共培养☆张勇，赵建宁。韩宁波—__●■l_■■—___—__—●-_●————●—l———●—————__一II一————IIIII●■●一Co-cultureofadiposederivedstemcellsandchondrocytesZhangYong．

2、ZhaoJian-ning，HanNing-bof)eDa巾T橹ntofOrthopaedics．NanjingGeneraIHospitalofNanjlngMilitaryAreaCommandofChinesePLA，SecondMiIhryMedicalUniversityofChinesePLA．Nanjing210002．JiangsuProvince．ChinaZhangYong☆．＆udyingfordoctorate．Attendl叼physjcian．DepartmentofOrthop

3、aedics．NanjingGeneralHospitalofNanjlngMilitaryAreaCommandofChinesepLASecondMilitaryMedicaIUniverailvofChinesePLA．Nanjing210002,JiangsuProvince．ChinaCorrespondenceto：ZhaoJian-ning．Ma8Ier．Professor,DoctoraIsupervisor,DepartmentofOrthopaedics．NanjingGeneralHo

4、spitalofNanjingMilitaryAreaCommandofChinesePLA，SecondMilitaryMedicaIUniversityofChinesePLA．Naniing210002．JiangsuProvince．Chinazhaojianning．0207@163cornReceived：2∞串12．∞^卿恻：2009-02-144632AbstractBACKGROUND：StudyshowsthatbonematToWmesenchyma}stemcellscanbeind

5、ucedintochondrocytesinthemicroenvironmentofarticularcartilage．0BJECTIVE：TovedfYthefeasibilityofinducingadiposededvedstemcells(ADSCs)intochondrocytassoastoprovideaRoveIapproachforarticularcartilagetissueengineering．DESlGN．TIMEANDSETTING：ArandomizedcalItrial

6、wasperformedattheStateKeyLabOratoryofPharrnaceuticaIBiotechnology．NaniingUniversityfromSeptember2007toJuly2008．MATER睢LS：AdultNewZealandwhiterabbitsofcleangrade，weighing1．5Io2．Okg，ofeithergender．METHODS：PrimarvADSCsandaficutarchondrocytesfromNewZealandwhita

7、rabbitswereisolatedandthenseededon6一poreplatesandmillicelIcultureplateinsertsseparately．Aft：er2weeksofco-culture．trypsinizationwasferminatedandADSCsweredetectedwithoiIredOstainingmethod．alkalinephosphatasedetectionmethodandtoluidinebluestainingmethodrespec

8、tively．MAINOUTCOMEMEASURES：ThecytomorphologyofADSCswasobservedunderinvertedmicroscopebeforeandafterco-culturerespectively．Afterco-culture。theproteoglycanexpressionofADSCswasdetectedwithtoluidinebluestainingme