硫酸软骨素酶abc聚乳酸聚乙醇酸共聚物缓释微球的制备及其体外性质

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1、万方数据硫酸软骨素酶ABC-聚乳酸．聚乙醇酸共聚物缓释微球的制备及其体外性质冰☆张德盛”，钟德君’，宋跃明1，吴江3Preparationandinvitropropertiesofcontrolledreleasepoly(1actic-co-glycolicacid)microspheresincorporatingchondroitinaseABCZhangDe—shen91”，ZhongDe-junl，SongYue-min91，WuJian93AbstractBACKGRoUND：ChondroitinaseABCpromote

2、stheoutgrowthofaxonsbydisruptionofchondroitinsulphateproteoglycans．whichisexpressedinthe10calsiteofspinalcordinjury．Itisnecessarytomultipleadministrationsorcontrolledreleaseofchondroitinaseforitsunstability．OBJECTIVE：TopreparethechondroitinaseABCmicrosphereswithcontrolled

3、releasepoly(1acfic-co—glycolicacid)(PLGA)，andobservethegeneralpropertiesinvitro．DESIGN，TIMEANDSETTING：RepeatedmeasurementtrialswereperformedinChengduOrganicChemicalsCo．。Ltd．。ChineseAcademyofSciencesandPharmacologyLaboratoriesofWestChinaCenterofMedicalScienceofSichuanUnive

4、rsityfromMarch20()6toJanuary2007．MATERIALS：PLGA，chondroitinaseABC，CH2C12andcondroitinB．METHoDS：ChondroitinaseABC—PLGAmicrosphereswerepreparedwithdoubleemulsionmethods．11豫saltsolutionsWCl-etakenasthemediumofthedrugreleaseinvitro．MAINoUTCoMEMEASURES：Themorphousofchondroitin

5、aseABCPLGAmicrosphereswasobservedunderseanningelectronmicroscope．山eparticlediameterdistributionsweremeasuredusingMalvemlaserparticlesizeanalyzer．thedrugloadingrateandencapsulationrateofthemicrospheresweredetectedusingenzymatichydrolysisspectrophotometry，andthedrugreleasec

6、urveinvitrowasdrawnatdays0．5，1，1．5。2，3，4，6，8，lO，12，14，16，18and21．RESULTS：nemorphologyandtheparticlesizedistributionofthechondroitinaseABCmicrospheresweregood．withameandiameterof5．538umandaspanof1．479．Theaveragedrugloadingwas(15．55_-+0．90)％，theaveragedrugencapsulationratew

7、as(53．88+1．45)％：n抡cumulativedmgreleaseratiowas0．85l4wit}lin3weeks，andwithastabledrugreleasepropertiesandfitfortheWeibullequations：lnln(1门一n=0．73961—卜1．617，尺。=o．9933．CoNCLUSIoN：ThechondroitinaseABCmicrospheresexhibitwell—distributedmorphology．UalTOWparticlesizedistribufion

8、andgoodredispersibility，theyCallmaintainalltherapeuticconcentrationwithin3weeks．ZhangDS，ZhongDJ．