同种异体气管软骨脱细胞基质构建组织工程化气管的初步研究

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1、()200946ChinJInjuryRepairandWoundHealing(ElectronicEdition),December2009,Vol4,No.66431981MSCsMSCsMSCsMSCs42.482.32g/ml19.621.30g/mlp<0.01HE1207MSCs14Experimentalstudyonconstructionoftissue-engineeredtracheawithallogenicacellularmatrixoftrachealcartilageLISha

2、ng-binYUQing-pingZHANGWen-haoHUORan.Departmentofcosmetologyandplasticandburn,ProvincialHospitalaffiliatedtoShandongUniversity,Jinan250021,China.Y2004C14250021274000272000Emailhuoran@medmail.com644()200946ChinJInjuryRepairandWoundHealing(ElectronicEdition),De

3、cember2009,Vol4,No.6CorrespondingauthorHUORanEmail:huoran@medmail.com.cnAbstractObjectiveToexploretheapplicationvalueoftheallogenictrachealacellularmatrixintheconstructionoftissue-engineeredtrachea.MethodsTheallogenictrachearingswereprocessedbyimproved-Court

4、manmethodtoremoveallcellsinthetissue.ThechondrogenicinducedMSCstakenfromhostcanineswereculturedwithallogenictrachealacellularmatrixinvitroandinvivotoobservetheadherenceandproliferation.ResultsAllcellsintheallogenictracheaswereremovedcompletelybyimproved-Cour

5、tmanmethodwithoutdamagetothestructureofthetrachealacellularmatrix.TheinducedMSCssurvivedandproliferatedonthesurfaceofmatrixinashortperiod.Butthecellsfailedtogrowintotheinternalpartofthematrixandformnewcartilagetissue.ConclusionsTheimproved-Courtmanmethodcanr

6、emoveallcellsofthetracheasamplescompletely.Butthefeaturesofvoidratioandperforatingabilityneedtobefurtherimprovedbeforetheallogenictrachealacellularmatrixisusedforthescaffoldmaterialoftissue-engineeredtrachea.KeywordsCanines;AcellularMatrix;TrachealCartilageT

7、issueengineering3cm5[1,2][3]Mesenchymalstemcells,MSCsMSCs4Beagle6(70.5kg1416ml1.073g/mlPercollMSCs315%FBSL-DMEM10ng/mlTGF-110ng/mlIGF-()200946ChinJInjuryRepairandWoundHealing(ElectronicEdition),December2009,Vol4,No.664537.5g/mlC6.25g/mlGAG1414-6246-10PBS1%Tr

8、ixton2448721206[4]2Courtman:A:1000ml6.05gTris0.2gEDTA10kIUAprotininpH8.0B1000ml6.05gTris87.7gNaCl0.2gEDTA10KIUAprotininpH8.0C1000ml10mlTritonX-1000.2gEDTA10kIUAprotininpH7.4D1000ml1.21gTrisDNAse