医学毕业论文可注射性同种异体工程化软骨的构建

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1、可注射性同种异体工程化软骨的构建姓名：2014年6月25日可注射性同种异体工程化软骨的构建作者：罗家胜李贵泽崔鹏程高鹏飞【关键词】软骨关键词：软骨;软骨细胞;生物医学工程;生物相容性材料摘耍:目的研究在有免疫力的动物体内形成同种异体工程化软骨的可行性;并验证本实验使用的生物材料聚氧化乙饰两飾凝胶(Copolymergelofethyleneandpropyleneoxide)在组织工程研究屮应用的可行性.方法无菌条件卜'取新主.兔关节软骨，经II型胶原酶消化，将软骨细胞和生物材料复合成细胞浓度为5x107ml-1的

2、复合物并移植于兔皮下，同时设立单独注射细胞和材料两个对照组.分别于4,6,8，12wk取材行大体观察，石蜡切片HE染fe,SafraninO染fe,Masson’s三色染色，了解软骨形成情况.结果2wk后即可于皮下触及新生结节，4wk后取材即有基质分泌及胶原形成.于6wk左右软骨接近成熟，生物材料基本吸收.8,12wk软骨基木接近正常软骨.实验中未见明显的免疫排斥反应.而对照组未见软骨形成.结论该实验使用的生物材料具有良好的生物相容性，可降解性，无细胞毒，是较理想的组织工程生物材料;应用该实验的方法可在有免疫力的动

3、物体内形成同种异体工程化软骨，这为临床某些美容和重建手术提供了一种微创而可塑型的可能的手段.Keywords:cartilage;chondrocytes;biomedical-engineering;biocompatiblematerialsAbstract:AIMTostudythefeasibilityoftheformationofallogeneictissueengineeredcartilageinimmunocompetentan-imalandtoverifythatthematerialuse

4、dinourexperimenthastheidealfeaturesastissue-engineeringbiomaterial.METHODSFreshnewbornrabbits'articularcartilageswereobtainedundersterilecondition(6hafterdeath)andincu-batedinthesterile3gMtypeIIcollagenasesolution.Af-terdigestionof8〜12h，thesolutionwasfilteredt

5、hroughal50micronnylonmeshandcentrifuged，thenthechondro-cyteswerewashedtwicewithphosphatebufferedsaline(PBS)andmixedwiththebiomaterialtocreateafinalcelldensityof5xl07ml-1.Theresultingcell-biomaterialadmix-turewasinjectedsubcutaneously0.3mleachpoint，andthecontro

6、lgroupswereinjectedwithonlythebiomaterialorthesuspensionofchondrocytewiththedensityof5xl07ml-1.After4,6,8andl2weeks，theneocartilageswereharvestedforanalysis.RESULTSWetouchedthenewnodessubcuta-neouslyafter2wk.Inthesectionsofthesamplesharvestedafter4wk,wefoundth

7、atthematrixwassecretedandthecollagenformed.After6wk，theneocartilageswerematureandthebiomaterialwasalmostcompletelydegraded.Intheexperimentgroupsweobservednoobviousimmunerejectionresponse.Onthecontrary，inthecontrolgroupstherewerenoneocartilageformed.CONCLUSIONB

8、iomaterialusedinourexperimentisarelativelyidealtissue-engineeringbioma-terial.lt’sfeasibletoformallogeneictissueengineeredcarti-lageinanimmunocompetentanimalthatprovidesanalterna-t