兔关节内积血对关节软骨影响的实验研究

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1、中国康复医学杂志，2007年，第22卷，第1期ChineseJournalofRehabilitationMedicine,Jan.2007,Vol.22,No.131.墓础研究.兔关节内积血对关节软骨影响的实验研究张继春‘陈金栋‘陈百成‘田德虎‘摘要目的:探讨短期关节内积血对关节软骨基础研究的影响。方法:新西兰大白兔36只随机分为2组。A组:右后膝关节内注人自体静脉血4m1，左后膝关节作为空白对照，在第3天重复一次，剂量同前。B组:右后膝关节内注人自体静脉血4m1，左后膝关节2m1，其余步骤与A组完

2、全相同。分别于第1周、4周、8周取材，测定水、胶原含量的改变，同时进行大体及光镜观察。结果:①受出血影响，第1,4周含水率都明显增加(均为P<0.05)，第8周时与对照组比较无明显差异(P>0.05)。胶原含量在第I周低于对照组(P<0.05),4ml组低于2ml组(P<0.05)。第4,8周与对照组比较均无明显差异(P>0.05),O2HE染色结果:实验侧软骨表面不光滑，细胞排列欠整齐，潮线变模糊。4m1侧比2m1侧变化明显。结论:不同量的关节内出血对关节软骨的影响不同，量大者对含水率、蛋白多糖、胶

3、原的影响大于量小者。关键词关节软骨;关节内积血;含水率;胶原中图分类号:1684,149文献标识码:A文章编号:1001-1242(2007)-01-0031-03Experimentalstudyonblood-induceddamagetoarticularcartilageinhemarthrosis/ZHANGJichun,CHENJindong,CHENBaicheng,etal.//ChineseJournalofRehabilitationMedicine,2007,22(1):31-3

4、3AbstractObjective:Toexploretheblood-induceddamagetoarticularcartilageinhemarthrosis.Method:Thirty-sixrabbitswererandomlydividedinto2groups.IngroupA,freshautologousblood(4m1)wasinjectedintotherightkneeposteriorlyatday1.Thesamevolumeofbloodwasinjectedint

5、othesamekneeatday3.Contralateral(left)kneewerenotinjectedatalltoactascontrol.IngroupB,4m1and2m1bloodwasinjectedintorightkneeandleftkneerespectivelytoobservethedestructiononcartilagebydifferentamountofblood.Theanimalsofeachsub-groupweresacrificedatthe1st

6、,4thand8thweekrespectively.Thecontentsofwaterandcollagenwerecompared,andthechangesofpathologywereobserved.Result:①Watercontentincreasedsignificantlyatthe1stweekand4thweekcomparedwiththecontrolgroup(P<0.05).Therewasnodifferenceofwatercontentatthe8thweek(

7、P>0.05).Thecollagencontentwaslowerthanthecontrolatthe1stweekandhigheratthe4weekand8thweekthanatthe1stweek(P<0.05).Thecollagencontentin4m1groupwaslowerthanthatin2m1groupatthe1stweek(P<0.05).Therewasnodifferencebetweentwogroupsatthe4thweekand8thweek(P>0.0

8、5).②Lightmicroscope(HE)observation:Thearticularcartilageofexperimentalsidebecameroughwithmoredivisioncells.Thetidemarkbecameunclear.Alloftheseappearancesweremoreapparentin4m1groupthanin2m1group.Conclusion:Differentamountsofbloodr