壳寡糖及其衍生物对体外培养成骨细胞增殖的作用特点

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1、万方数据壳寡糖及其衍生物对体外培养成骨细胞增殖的作用特点术水☆金黎明1，魏长征2，田文杰1，赵小菁1，王雅玲‘。范圣第1■——■●■———■————__——■_i一——————————i———■■■———————■————■——●■●■—■●ChRooligosaccharideanditsderivativespromotetheprofiferationofosteoblastsinvitroJinLi．ruin91，WoiChang·zhen92，"nanWen-jiel，ZhaoXiao-ji

2、n91。WangYa-lin91，FanSheng-dilAbstractBACKGRoUND：Cllitooligosaccharide(COS)andmonosaccharidemolecularsderivedfromchitinandchitosanhavethesuperioritytomacromolecularsaccharide．owingtolowweightandgoodwatersolubility．OBJECTIVE：ToobservetheabilitiesofCOSandi

3、tsderivates，suchasglucosamine(GIuNH2)，N—acetyl-glucosamine(NAG)andcarboxymethyl—COS(CM—COSltopromotetheproliferationofosteoblastsinvitro．DESIGN．TIM[EANDSETnNG：ControlledobservationexperimentswerecompletedintheBiochemistryLaboratory，CollegeofMarineLifeSc

4、iences．OceanUniversityofChinafromFebruary2004toJuly2005．A

5、值TERIAI．S：cellstrainMC3T3-E1ofmiceskul／wasofferedbytheCenCenterofPekingUnionMedicalGollegeHos#嘲(China)．NAG，COS，GIuNH2andCM—COSwerepreparedintheBiochemistryLaboratory，CollegeofMarineLifeSciences，O

6、ceanUniversityofChina(China)．M【ETHODS：NAG，COS，GluNH2andCM．COSwereaddedintoDMEMmediumaccordingtotheconcentrationsof10，100，500，1000and2000mg／L．respectively．ThemRNAwasextractedfromtheculturedosteoblasts．ThentuRNAdifferentialdisplayanalysiswasappliedtostudy

7、themechanism．M渔INoUTCoM咂M匮ASURES：TheproliferationofosteoblastswasstudiedbvMTrmethod．11碡growthofosteoblastswasobservedunderinvertedmicroscope．ThemechanismwasstudiedbymRNAdifferentialdisplay．RESULTS：NAGatappropriateconcentrationscouldpromotetheproliferati

8、onofosteoblasts．andthehigherconcentration(1o()0mg／L)hadstrongereffects．ThepromotionofCOSwasmoreobvious，andthemosteffectiveconcentrationwas500mg／L．GluNH2pmmotedtheproliferationofosteoblastson10Wel"concentrations(10and100meCL)whilehigherconcentrations(>50

9、0me／L)indicatedinhibitingfunction．CM．COSalsoshowedthefunctionofproliferation，buttheeffectwasnotprominent．nleopticalmicroscopyoutcomewasconsistentwiththatofMTTmethod．TheresultsofmRNAdifferentialdisplayshowedthatfoiffsampleshadnoob