欢迎来到天天文库
浏览记录
ID:18018682
大小:249.48 KB
页数:3页
时间:2018-09-12
《丹参逆转胃癌耐药细胞的作用及其机制分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据·4054·现代中西医结合杂志ModemJournalofIntegratedTraditionalChineseandWesternMedicine2009Nov,18(33)丹参逆转胃癌耐药细胞的作用及其机制分析玄基泽1,关宏伟1,韩(1.大连医科大学附属第一医院,辽宁大连116011;2.陕西西安710032)辉1,乔太东2第四军医大学西京医院全军消化病研究所,【摘要】胃的探讨丹参对人胃癌耐药细胞系SGC7901/ADR耐药性的逆转作用及其机制。方法以不同浓度的丹参(SAL)处理耐药系
2、SGC7901恤R及敏感系SG凹90l细胞,四甲基偶氮唑盐(MTT)比色法检测丹参对两种细胞系的毒性及丰数却帝J率(IG0)。漉式细胞仪(FCM)检测丹参对两种细胞内ADM浓度和细胞周期的影响,SP法免疫组化染色检测两种细胞P一糖蛋白(P—gp)争DNA拓扑异构酶Ⅱ(TopoII)的表达。蛄果丹参的非细胞毒性药物剂量为500mg/L以下;丹参可逆转SGC7901/ADR细胞对ADM的耐药性,逆转倍数为1.89(P<0.01);提高细胞内ADM浓度,ADM增加1.55倍(P3、阻滞于Gl、G2期;对细胞膜P—gP的表达轻度下降、TopoII的表达略有增加。但无显著性差异(P均>O。05)。砖论丹参对SG凹901和SGC7901/ADR细胞无杀伤作用。其执毒J是丹参能增加对药细胞内化疗药物浓度,使DNA合成期(S期)细胞含量下降。[关键词】丹参;胃癌;逆转;SCK77901/ADR细胞[中图分类号】R965.2[文献标识码】A[文章编号】1008—8849{2009)33—4054一03EffectandrelatedmechanismofSalviamiltiorrhiz4、aonhumandrug-resistantgastriccancarcellXuanJizel,GuanHongweil,HanHuil,QiaoTaidon92(1.TheFirstAffiliatedHospitalofDalianMedicalUniversity,Dalian116011,Liaoning,China;2.XijingHospitalofTheFourthMilitaryMedicalUniversity,Xi’an710032。Shaanxi,China)Abstract5、:ObjectiveItistOapproachtheeffectsandrelatedmechanismofSalviamiltiorrhiza(SAL)onreversethemul—tidrugresistance(MDR)ofgastriccarcinomacelllineSGC790l/ADRtOadriamycin.MethodsDrugresistanceSGC7901/ADRceiIsanddrugsensitiveSGC7901eellsweretreatedwithSALofse6、veralconcentrations.TheuncytoxicitydosesandIC50ofthetwoeelllinesweredetectedbyMTrassay;eellcycleandADMconcentrationinthecellsweredetectedbyflowcytometry;theexpressionofP-glycoprotein(P—gp)andtopoisomeraseⅡ(Topo1I)ingastriccancercellsweredetectedbyimmun7、ohistoehemicalstainingtechnique.ResultsTheuncytotoxicitydosesofs虬WaS500mg/L.IC50vaIuesofADMofSGC7901/ADRcellswerereducedsignificantly,reversalindex(RI)WaS1.89(P<0.01)。andADMconcentrationinSG凹901/c气DRcellsWaSupregulated,upregulatedmultipleWas1.55(P<0.058、).SALmadecellcycleblockedinGI,G2phase.TheexpressionofP—gpincellmembranereducedslightlyandtheexpressionofT000Ⅱincreasedalittle,butthereWasnosignificantdifference(P>O.05).ConclusionSALhasnoeffectonSGC7901/ADRanddrugsensitiveSGC7901celllin
3、阻滞于Gl、G2期;对细胞膜P—gP的表达轻度下降、TopoII的表达略有增加。但无显著性差异(P均>O。05)。砖论丹参对SG凹901和SGC7901/ADR细胞无杀伤作用。其执毒J是丹参能增加对药细胞内化疗药物浓度,使DNA合成期(S期)细胞含量下降。[关键词】丹参;胃癌;逆转;SCK77901/ADR细胞[中图分类号】R965.2[文献标识码】A[文章编号】1008—8849{2009)33—4054一03EffectandrelatedmechanismofSalviamiltiorrhiz
4、aonhumandrug-resistantgastriccancarcellXuanJizel,GuanHongweil,HanHuil,QiaoTaidon92(1.TheFirstAffiliatedHospitalofDalianMedicalUniversity,Dalian116011,Liaoning,China;2.XijingHospitalofTheFourthMilitaryMedicalUniversity,Xi’an710032。Shaanxi,China)Abstract
5、:ObjectiveItistOapproachtheeffectsandrelatedmechanismofSalviamiltiorrhiza(SAL)onreversethemul—tidrugresistance(MDR)ofgastriccarcinomacelllineSGC790l/ADRtOadriamycin.MethodsDrugresistanceSGC7901/ADRceiIsanddrugsensitiveSGC7901eellsweretreatedwithSALofse
6、veralconcentrations.TheuncytoxicitydosesandIC50ofthetwoeelllinesweredetectedbyMTrassay;eellcycleandADMconcentrationinthecellsweredetectedbyflowcytometry;theexpressionofP-glycoprotein(P—gp)andtopoisomeraseⅡ(Topo1I)ingastriccancercellsweredetectedbyimmun
7、ohistoehemicalstainingtechnique.ResultsTheuncytotoxicitydosesofs虬WaS500mg/L.IC50vaIuesofADMofSGC7901/ADRcellswerereducedsignificantly,reversalindex(RI)WaS1.89(P<0.01)。andADMconcentrationinSG凹901/c气DRcellsWaSupregulated,upregulatedmultipleWas1.55(P<0.05
8、).SALmadecellcycleblockedinGI,G2phase.TheexpressionofP—gpincellmembranereducedslightlyandtheexpressionofT000Ⅱincreasedalittle,butthereWasnosignificantdifference(P>O.05).ConclusionSALhasnoeffectonSGC7901/ADRanddrugsensitiveSGC7901celllin
此文档下载收益归作者所有