丹参对大鼠移植肝脏再灌注损伤的防护及肝细胞凋亡的影响

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1、万方数据·870·南方医科大学学报(JSouthMedUniv)2008；28(5)丹参对大鼠移植肝脏再灌注损伤的防护及肝细胞凋亡的影响王宇1，周杰1。蒋晓青2(南方医科大学南方医院1肝胆外科，2麻醉科ICU，广东广州510515)摘要：目的研究含丹参冷灌注液对移植肝缺血再灌流损伤的防护及肝细胞凋亡的影响。方法将40只SD大鼠分为对照组、丹参组及假手术组，建立原位肝移植模型．在供肝灌注冷保存时，以4℃乳酸林格氏液为基液，丹参组灌注保存液中加丹参注射液(60ml／L)，对照组不加丹参。保存5h后置人受体。移植后6h处死大鼠取样，检测血清中AST、JU

2、J及肝组织中氧自由基代谢相关指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH．PX)水平，采用TUNEL法检测移植术后肝细胞凋亡情况，光镜及透射电镜下观察移植肝脏组织形态学及超微结构的改变。结果移植肝再灌注后丹参组AST、ALT显著低于对照组。与对照组相比，丹参组肝细胞凋亡指数明显下降(氏0．01)，肝组织中MDA含量较对照组降低(氏0．01)，SOD、GSH．PX活性较对照组升高(P

3、．再灌注损伤有保护作用。关键词：丹参；肝移植；再灌注损伤；大鼠；细胞凋亡中图分类号：R363．文献标识码：A文章编号：1673-4254(2008)05-0870．04EffectsofRadixsalviaemiltiorrhizaeonreperfusioninjuryandhepatocyteapoptosisindonorliverafterorthotopiclivertransplantationinratsWANGYu。，ZHOUJiel，JIANGXiao-qin921DepartmentofHepatobiliarySurgery

4、,ZDepartmentofAnesthesia，NanfangHospital，SouthernMedicalUniversity，Guangzhou510515，ChinaAbstract：0bjectiveToinvestigatetheeffectofRad／xsalviaemihiorrhizaeasacoldreperfusionsolutionondonorliverischemia—reperfusioninjuryandhepatocyteapoptosisinratmodelsoforthotopiclivertransplan

5、tation．MethodsFortymaleSDratswererandomlydividedintomiltiorrhizaegroup，controlgroupandshamoperationgroup．ForcoldpreservationofthegraR，miltiorrhizae-containingRinger'slactatesolutionwasusedasthereperfusionandpreservationsolutioninthemiltiorrhizaegroup，andonlyRinger'slactatesolu

6、tionwasusedinthecontrolgroup．Allthegraftswerepreservedfor5hbeforeorthotopictransplantion．Sixhoursaftergraftreperfusion，therecipientratsweresacrificed，andtheserumalanineaminotmnsfemse(ALT)andaspartateaminotransferase(AST)weremeasured,andthechangesofmalondialdehyde(MDA)，superoxi

7、dedismutase(SOD)，andglutathioneperoxidase(GSH—Px)inthelivergraftwereobserved．ThehepatocyteapoptosiswasdetectedusingTUNEL，withthehistopathologicalchangesobservedunderlightandelectronmicroscopes．ResultsSerumALTandASTlevelsweresignificantlylowerinmiltiorrhizaegroupthaninthecontro

8、lgroupaftergraftreperfusion(P，o．01)．Comparedwiththeshamoperat