沙漠干热环境下猪腹部肠管火器伤后肝组织中NFκB 、TNFα的表达.doc

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1、沙漠干热环境下猪腹部肠管火器伤后肝组织中NFκB、TNFα的表达作者：刘江伟，吕月涛，薛会朝，李泽信，张永久，张东，雷涛【摘要】　　目的探讨沙漠干热环境下猪腹部肠管火器伤后肝脏组织中核因子κB（NFκB）和肿瘤坏死因子α（TNFα）的表达及其与肝损伤的关系。方法健康长白仔猪42头随机等分为对照组和伤后1、2、4、8、12h和24h组，在沙漠干热环境下建立腹部肠管火器伤模型后，用免疫组化及图像分析法测定各组肝脏组织内NFκB和TNFα的表达，同时测定血清中ALT水平，在光镜下观察各组肝脏组织学变化。

2、结果伤后各组肝内NFκB、TNFα表达明显高于对照组(P＜0.05)，NFκB于伤后1h和4h出现2个高峰(P＜0.05)；TNFα于伤后1h和8h出现2个高峰(P＜0.05)；血清ALT水平在伤后明显升高，也于伤后1h和8h出现2个高峰(P＜0.05)；伤后1、2h组出现逐渐加重的肝细胞水肿、变性，伤后4h组开始出现逐渐加重的肝细胞点状坏死、灶状坏死和炎细胞浸润，对照组光镜下未见明显的损伤性变化；NFκB与TNFα的表达在2h组和4h组均有正相关关系（P＜0.05）。结论沙漠干热环境下腹部肠管火器伤

3、后肝内NFκB、TNFα表达一致性升高，二者在伤后早期表达密切相关，并与肝脏结构和功能损伤变化基本一致，提示NFκB和TNFα在沙漠干热环境下腹部肠管火器伤后肝损伤过程中起重要作用。【关键词】腹部火器伤肝损伤干热环境沙漠核因子κB肿瘤坏死因子α　　　Abstract：ObjectiveToexploretheexpressionofnuclearfactor(NF)κBandtumornecrosisfactor(TNF)αinhepatictissueandthemechanismofliverin

4、juryfollowingintestinalperforationduetoabdominalfirearmwoundindryheatenvironmentofdesert.MethodsAtotalof42ChangBaipigletswereequallydividedinto7groupsatrandom:controlgroup,andpostwounded1hour(h)group,2hgroup,4hgroup,8hgroup,12hgroupand24hgroup.Themod

5、elsofintestinalperforationduetoabdominalfirearmwoundwereestablishedinthedryheatenvironmentofdesert.ExpressionofNFκBandTNFαwasmeasuredwithimmunohistochemistryandimaginganalysisinallthegroups.Serumalanineaminotransferase(ALT)levelswerealsodeterminedatthesame

6、time.Thealterationofhepatictissuewasobservedunderlightmicroscope.ResultsExpressionofhepaticNFκBandTNFαinthepost-woundedgroupswassignificantlyelevated,comparedtothatofthecontrolgroup(P<0.05);NFκBpresentedtwopeaksin1hand4hgroups;TNFαappearedanditstwope

7、aksbelongedto2hand8hgroups.LevelsofserumALTwerealsomarkedlyincreased,andthetwopeakswerein2hand8hgroup(P<0.05).Inallthe5measurements,thesecondpeakexceedthefirstone(P<0.05).Underlightmicroscope,hepatocellularedemaandmetamorphosiswasgraduallyaggravated

8、from1hgroupto2hgroup.Hepatocellularspotnecrosisandpiecemealnecrosiswereobservedin4hgroup,andincreasedfrom4hgroupto24hgroup.Thelivermorphologyofthecontrolgroupwasnormalunderlightmicro