TNFα在沙漠干热环境下猪腹部肠管火器伤后肝组织中的表达.doc

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1、TNFα在沙漠干热环境下猪腹部肠管火器伤后肝组织中的表达作者：刘江伟　张永久　李泽信　吕月涛　张东　雷涛　冯德元【摘要】　　目的探讨沙漠干热环境下猪腹部肠管火器伤后肝脏组织TNFα的表达和肝功能的变化。方法沙漠干热环境组和常温环境组健康长白仔猪各42头随机等分为对照组和伤后1、2、4、8、12h和24h组，实验组建立腹部火器伤肠管穿透模型后，用免疫组化及图像分析法测定各组肝脏组织内TNFα的表达，同时测定血清中AST水平。结果伤后各组肝组织TNFα表达明显高于对照组，沙漠干热环境组于伤后2h和8h出现2个高峰；常温环境组于伤后2h和12h出现2个高峰。血清AST水平

2、于伤后显著增高，并于伤后2h出现第1个高峰，沙漠干热环境组和常温环境组分别于伤后8h和12h出现第2个高峰。结论沙漠干热环境下腹部肠管火器伤后肝脏TNFα的表达增加并与AST的变化趋势一致，而且峰值较常温组提前出现，提示TNFα在腹部肠管火器伤后肝损伤过程中可能起重要作用。【关键词】腹部火器伤；肝损伤；干热环境；沙漠；肿瘤坏死因子－α　　　Abstract：ObjectiveToinvestigatetheexpressionoftumornecrosisfactor(TNF)αinhepatictissueandthechangeinhepaticfunctioni

3、npigwithintestinalperforationduetoabdominalfirearmwoundindryheatenvironmentofdesert.MethodsAtotalof84Changbaipigletsweredividedequallyintonormaltemperatureenvironmentgroupanddryheatdesertenvironmentgroup.Eachgroupwasequallydividedintosevensubgroupsrandomly:controlgroupandwounded1hourgr

4、oup,2hourgroup,4hourgroup,8hourgroup,12hourgroup,and24hourgroup.Themodelofintestinalperforationduetoabdominalfirearmwoundwasestablishedinthewoundedgroups.ExpressionofTNFαwasmeasuredwithimmunohistochemistryandimageanalysisinallthegroups.SerumASTlevelwasalsodeterminedatthesametime.Resu

5、ltsTheexpressionofhepaticTNFαinthewoundedgroupsweresignificantlyelevatedwhencomparedwiththecontrolgroups.Itappearedtoreachtwopeaksin2hourgroupand8hourgroupinthedryheatdesertenvironmentgroup,andanothertwopeaksin2hourgroupand12hourgroupinthenormaltemperatureenvironmentgroup.Levelsofser

6、umASTinthewoundedgroupsweresignificantlyelevatedwhencomparedwiththecontrolgroups.ThedryheatdesertenvironmentgroupappearedtohavetwoASTpeaksat2hourgroupand8hourgroup,andthenormaltemperaturegroupappearedtohavetwoASTpeaksat2hourgroupand12hourgroup.ConclusionTheexpressionofTNFαinhepaticti

7、ssueinpigswithintestinalperforationduetoabdominalfirearmwoundindryheatdesert5environmentissignificantlyelevatedandaccompaniedwithdamageofhepaticfunction.ThepeaksofTNFαandASTappearearlierindryheatdesertenvironmentthaninnormaltemperatureenvironment,whichsugge