利用gus基因瞬时表达探索佛手叶盘基因枪转化参数

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1、利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数利用GU

2、S基因瞬时表达探索佛手叶盘基因枪转化参数利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数北机物报.2OO5.25(11):21,l521ActaBot.Borea1.一0ccident.Sin.文章编号:1000,1025(2005)11—211()6利用GUS基因瞬时表达探索佛手叶盘基因枪转化参数周春丽.,郭卫东,王德解,余初浪,路梅,李玉萍(Ii1科技{『lIi范:院命科,院.1}=『H3300I3;2浙jI~Ihli范人’:命环境科’院.浙{l金华;321001;3北农林科技人M岂院.陕畅陵I2Ioo)摘要:佛址乔科们橼类物

3、.r女r的约川和观赏价.flIJ.lf?币1较为t?.埘儿进遗f改良千¨币1?选行I分必婴.为.r将I枪转化法川J佛下的遗f々政良币¨币1?选f『.小实验以(IN为报I.利川l枪办法将J转入产佛丁外.jln过榆测(‘l,Vl佛rm?l?的瞬I1l『表达tl况.分析r外怵的幼嫩度,射,氦,r,,I次数等参数埘(SII=;:}lI,『的影响.似『究r山量榆测lll『I训.纳llJJ.以幼…为受体.射为6CFII,氦J为.581×10Pa.’￡为88.()16kl”条什卜.2dIII愉测(JI的小tt.fl;达f?..I次数多少埘(I瞬

4、II,J达影II阳4lIJJ关键词:佛r;IN枪;(I;瞬lll『中图分类号:Q785;Q9.72.7文献标识码:AExplorationoftheTransformationParametersoftheLeafDiscsofBergamotbyParticleBombardmentinVirtueofTransientExpressionofGUSGeneZH()UChun—li’...GU()Wei—dong,WANG1)e—jieYUChu—lang!.I.UMei!.IAYu—ping(ICollegeof1.ifeSc

5、iences.JiangxiNormalUniversityofScienceandTeehnology.Nanchang,Jiangxi3300I3,China:2Academyor1ireandEnvironmentScience.ZhejiangNormalUniversity.Jinhua,Zhejiang32IO01.(‘hina:3HorticultureCollege.NorthwestSciTechUniversityofAgricuhureandForestry.Yangling.Shaanxi7I2IO0,Ch

6、ina)Abstract:BergamotisaplantofhighmedicinalandornamentalvaluesthatbelongstoCitt—ttSmedi~,”I.var.sarcoda~’tylis(Noot.)Swingle,butdoesnothavemanyvarieties.Thusitisnecessarytoconductitsgeneticimprovementandvarietybreeding.Inordertoapplyparticlebombardmenttothegeneticimp

7、rovementandvarietybreedingofbergamot,thisstudy,usingGf,Sgeneasthereportgene.transformedGgeneintotheexplantsofJinhuabergamotbyparticlebombardment;thetransientexpressionofG己,Sgeneintheleafdiscsofbergamotwereexaminedtoanalyzetheeffectsofexplanttenderness,gunshot,heliumpr

8、essure,vacuumdegreeandbombardmentfrequencyontheexpressionofGUSgene,andexploretheoptimalexami—nationtime.Theresultsrevealedth