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1、基因枪转化的bar基因表达盒在水稻中遗传行为复杂JournalofGeneficsandGenomics(FormerlyActaGeneticaSinica)September2007,34(9):824835Availableonlineatwww.sciencedirect.comScienceDirectbclencerectHereditaryBehaviorofbarGeneCassetteisComplexinRiceMediatedbyParticleBombardmentYanZhao,QianQian～,HuizhongWang,DanianHuang1.Col
2、legeofFood,BiologicalandEnvironmentalEngineering,Zh~iangGongshangUniversity,Hangzhou310035,China2.StateKeyLaboratoryRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou310006,China;3.LifeScienceDepartmentofHangzhouTeacherCollege,Hangzhou310036,ChinaAbstract:Particlebombardmenttransformation
3、usingminimalgenecassette(containingthepromoter,openreadingframeandterminator)isthenoveltrendinplantgenetictransformation.anditsusehelpstoalleviatetheundesirableeffectsofplasmidvectorbackbonesequencesontransgenicplants.Inthepresentarticle,studiesrelatedtothehereditarybehaviorofbargenecasseReinT
4、1toT3generationsofthetransgenicrice(OryzasativaL.)linestransformedbyparticlebombardmenthavebeendiscussed.These—lectablemarkerbargenecassettethatintegratedwiththericegenomehadmultiplecopiesandshowedcomplexsegregationbehav—iorsincludingthepresenceof'falsehomozygotes',withabnormalsegregationratio
5、srangingfrom35:1to144:1(Basta～resistant:sensitiveplants)intheirprogenies.Infiveoutoftenoriginaltransgeniclines,bargenecanbestablytransmittedasadominantgenetoself-pollinatedT2progeny.ThehomozygoteswereobtainedinthreetransgeniclinesinT1generationregardlessofthemulti—ple—copyintegrationpatternsof
6、bargene.Southernblottinganalysisshowedthatmultiplecopiesofbargenecassettewerelinked,whichformedtransgenearraysinthehostricegenome.Theauthorsalsoobservedstabletransmissionofintegrationpatternsofbargenecassette,asobtainedfromSouthernblottinganalysis,intheregularlysegregatedtransgenicricelinesand
7、lossofgeneinanirregularlysegregatedtransgenicline.ThesegregationbehaviorvariedamongthetransgenicprogeniesthatexhibitedsimilarSouthernhybridizationpatternsofbargene.Onthebasisoftheseresults,themultiple—copyintegration,genelost,andgeneexp
