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1、吡格列酮对大鼠痛风性关节炎滑膜细胞因子表达的作用l24TianjinMedJ,Feb2007,Vol35No2吡格列酮对大鼠痛风性关节炎滑膜细胞因子表达的作用黄火高王兆君张彦彬施海燕韩春光刘永学△摘要目的:研究过氧化物酶体增殖物激活受体~/(peroxisomeproliferator-activatedreceptorgamma,PPAR~/)激动剂吡格列酮.对大鼠急性痛风性关节炎滑膜表达细胞因子的调控作用,探讨其作用机制.方法:大鼠单侧踝关节注射人工尿酸钠晶体制作急性痛风性关节炎模型,于注射48h后取关节滑膜,以RT—PCR法检测吡格列酮(2Omg?kg?d..)口服给药对滑膜表
2、达肿瘤坏死因子ct(TNF—d),白介素1B(IL_1B),白介素1受体拮抗剂(IL—IRa),细胞因子诱导中性粒细胞化学趋化因子(CINC,相当于人的白介素8),干扰素T(IFN一)和白介素4(IL-4)的作用.结果:吡格列酮用药组滑膜表达TNF—d,CINC和IFN显着低于对照组,抑制率分别为81.7%,90.9%,65_3%;IL一4表达高于对照组,增加率为340.O%;IFN一L-4比值显着低于对照组,抑制率为92.6%.2组IL-I~和IL-1Ra的表达差别无统计学意义.结论:吡格列酮对急性痛风性关节炎的抗炎作用与抑制TNF—d和CINC的表达.并促使Thl/Th2平衡向T
3、h2为优势转化有关.关键词过氧化物酶体增殖物激活受体噻唑烷二酮类痛风细胞因子类大鼠EffectsofPioglitazoneonCytokineExpressionsinSynoviuminRatswithAcuteGoutyArthritisHUANGHuogao,WANGZhaojun,ZHANGYanbin,SHIHaiyan,HANChunguang,LIUYongxueDepartmentofEndocrinologyandRheumatology,NavyGeneralHospitalofPLA,Beijing100037,ChinaAbstractObjective:T
4、oinvestigatetheeffectsofpemxisomepmliferator—activatedreceptorgamma(PPART)agonist,pioglitazone.oncytokineexpressionsinsynoviuminratswitIlacutegoutyarthritisinducedbymonosodiumuratemonohydrateStO.Methods:ModelofacutegoutyarthritisWaSinducedwiththeinjectionof1mgofMSUintorats'anklejointcavity.Fort
5、y—eighthoursafterinjection,cytokinemRNAexpressionsincludingTNF-or,IL-I~,IL—IRa,CINC(cytokine—inducedneutrophilchemoattractant,equivalenttoIL一8),IFN一andIL--4inarthriticsynoviumofratsweredeterminedbyreversetranscriptionpolymerasechainreaction(RT-PCR).Results:TheexpressionsofTNF-ct,CINCandIFN一inar
6、thriticsynoviumfrompioglitazonetreatedratsweresignificandylowerthanthosefromcontrolgroup,withreducedratesof81.7%,90.9%and65.3%,respectively.TheexpressionofIL一4wasmuchhigherinsynoviumfromtreatedratsthanthatfromcontrolgroup.withaugmentedrateof340.0%.TheratioofIFNT/IL--4Wassuppressedtoanarithmetic
7、rateof92.6%.whilenodifferenceswerefoundinexpressionofIL一18andIL-1Ra.Conclusions:Pioglitazone,aPPARTagonist,mightexertanti—inflammatoryeffectsthroughrepressingtheexpressionsofTNF—dandCINC,andthroughshiftingtheThlfrh2balancetoaTh2cy
