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1、平调饮对原发性肝癌患者AFPmRNA、MAGE-1mRNA表达的影响作者：宋爱英,杨丹,曹德胜,李威,王丽芹【摘要】 目的研究平调饮对原发性肝癌患者甲胎蛋白(AFP)mRNA、黑色素瘤抗原-1(MAGE-1)mRNA表达的影响,探讨平调饮对原发性肝癌的抑癌基质。方法原发性肝癌患者60例,随机分为治疗组和对照组,分别给予平调饮和华蟾素注射液,治疗2个疗程,采用RT-PCR技术检测原发性肝癌患者治疗前后外周血AFPmRNA、MAGE-1mRNA的表达,并取30例健康志愿者血样作为参考对照,结果用SP
2、SS13.0软件统计。结果平调饮使原发性肝癌患者的AFPmRNA、MAGE-1mRNA表达明显降低,2组治疗前后比较差异均有统计学意义(P＜0.01),治疗后治疗组与对照组MAGE-1mRNA表达(灰度比)分别为0.07±0.01、0.13±0.02,组间比较差异有统计学意义(P＜0.01),治疗组作用优于对照组。结论平调饮能够明显降低原发性肝癌患者AFPmRNA、MAGE-1mRNA表达,对MAGE-1基因表达的降低作用优于对照组,说明抑制MAGE-1基因表达可能是平调饮抗肝癌机制的靶点之一。【
3、关键词】原发性肝癌；平调饮；黑色素瘤抗原-1基因；甲胎蛋白基因 Abstract：ObjectiveTostudythechangesofexpressionof11AFPmRNAandMAGE-1mRNAinhepatocellularcarcinoma(HCC)afteroralPingtiaoyintherapy,andexploreitsinhibitoryactiontoHCC.MethodsSixtypatientswithHCCwererandomlydividedintotrea
4、tmentgroup(n＝30)andcontrolgroup(n＝30).OralPingtiaoyintherapyandinjectionofHuachansuweregivenfortwocoursesofmedicaltreatmentstotreatmentgroupandcontrolgrouprespectively.RT-PCRwasusedtodetectAFPmRNAandMAGE-1mRNAexpressioninperipheralbloodofpatientsbefor
5、eandaftertreatment.Atthesametime,30healthyvolunteerswereselectedasthecontrol.ThedatawasanalyzedbystatisticsofsoftwareSPSS13.0.ResultsTheexpressionofAFPmRNAandMAGE-1mRNAweremarkedlydecreasedafteroralPingtiaoyintherapy.Therewassignificantdifferencesbetw
6、eenbeforeandaftertreatmentintwogroups(P＜0.01).ThebrightnessratioofthestrapofMAGE-1vsβ-actinaftertreatmentinthetreatmentgroupandthecontrolgroupwas0.07±0.01and0.13±0.02respectively(P＜0.01),theresultofthetreatmentgroupwasbetterthanthatofthecontrolgroupob
7、viously.ConclusionPingtiaoyincaneffectivelysuppresstheexpressionofAFPmRNAandMAGE-1mRNAinperipheralbloodofhumanHCC,itwasbetterthanthecontrolgroupindecreasingtheexpressionofMAGE-1mRNA.SuppressingtheexpressionofMAGE-1mRNAis11possiblyoneofthefunctiontarge
8、tsoftheanti-tumormechanismofPingtiaoyin. Keywords：hepatocellularcarcinoma；Pingtiaoyin；MAGE-1mRNA；AFPmRNA 原发性肝癌(HepatocellularCarcinoma)是世界十大恶性肿瘤之一,据统计,每年全球新患肝癌人数为62.6万人,因肝癌死亡者高达59.8万人,居死亡原因第3位,而新发肝癌病例中55%发生于中国,我国的肝癌诊治形势十分严峻[1]。手术切除目前仍是肝癌首选的、
