快速老化小鼠的学习记忆缺陷及其生化机制

《快速老化小鼠的学习记忆缺陷及其生化机制》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、快速老化小鼠的学习记忆缺陷及其生化机制【摘要】目的用快速老化小鼠(SAMP8)模拟老年痴呆，观察其学习记忆能力，并探讨其学习记忆能力下降的可能机制。方法采用10月龄SAMP8，并用同龄抗快速老化小鼠(SAMR1)作为正常对照。用水迷路检测近记忆以及空间学习记忆能力。用生物化学方法检测大脑皮层线粒体膜电位和ATP酶活力，观察线粒体功能；检测皮层和海马胆碱乙酰基转移酶(ChAT)和乙酰胆碱酯酶(AChE)的活力，观察中枢胆碱能神经功能；检测血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量，观察其全身性氧化应激状况。结果与SAMR1相比，SAMP8出现明显的衰老体征，

2、其近记忆和空间学习记忆能力明显减弱，具有衰老和痴呆特征；皮层线粒体膜电位和ATP酶活力显著降低，海马ChAT活力显著下降，AChE活力显著升高，血清SOD活力稍有增高。结论SAMP8可以很好地模拟老年痴呆，其机制包括大脑皮层线粒体功能降低、海马胆碱能神经功能下降以及氧化应激等。【关键词】快速老化小鼠老年痴呆线粒体胆碱能神经氧化应激　　ABSTRACT:ObjectiveTosimulateseniledementiawithsenescenceacceleratedmouse/prone8(SAMP8)andinvestigateitslearningandmemo

3、rydeficits,andtheirbiochemicalmechanisms.MethodsThegeneralstatusofSAMP8wasinvestigated18comparedwiththatofSAM/resistance1(SAMR1).Thentheirrecentmemoryandspatiallearningandmemoryabilitiesweredetectedwithwatermaze.Themitochondrialmembranepotentialandadenosinetriphosphatase(ATPase)ofcortex

4、weredeterminedwithbiochemicalmethodtoinvestigatetheirmitochondrialfunction.Thecholineacetyltransferase(ChAT)andacetylcholinesterase(AChE)activitiesofcortexandhippocampusweredetectedwiththeabovemethodtoobservetheircentralcholinergicnervefunction.Thesuperoxidedismutase(SOD)activityandmalo

5、ndialdehyde(MDA)contentinserumofmiceweredeterminedwithbiochemicalmethodtoobservetheirgeneraloxidativestress.ResultsComparedwithSAMR1,SAMP8displayedsignificantsenilephysicalsigns,andweakrecentmemoryandspatiallearningandmemorycapabilitiesweresimilartothosecharacteristicsofsenescenceanddem

6、entia.Meanwhile,themitochondrialmembranepotentialandATPaseactivityofcortex,andtheChATactivityofhippocampuswerelowerthanthoseofSAMR1,buttheAChEactivityofhippocampuswashigherthanthatofSAMR1,andtheserumSODactivitywasslightlyhigherthanthatofSAMR1.ConclusionSAMP8maysimulateseniledementiaandi

7、tspathogenesisinvolvesinsufficientcortexmitochondrialfunction,decreasedcholinergicnervefunction,18andoxidativestress.　　KEYWORDS:senescenceacceleratedmouse/prone8(SAMP8);seniledementia;mitochondrion;cholinergicnervefunction;oxidativestress　　快速老化小鼠(senescenceacceleratedmouse,SA