ck20mrna、cd44v6和pcna对大肠癌早期肝转移预测的研究

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1、CK20mRNA、CD44v6和PCNA对大肠癌早期肝转移预测的研究作者：陈磊周东风林惠忠李晓川李宏【摘要】目的：研究CK20mRNA、CD44v6和PCNA与大肠癌肝转移的关系，探讨临床预测大肠癌早期肝转移的客观指标。方法：应用荧光定量RTPCR法检测50例大肠癌患者回流门静脉血中CK20mRNA，同时应用免疫组织化学方法测定大肠癌癌组织中CD44v6、PCNA的表达，并与对照组比较。结果：大肠癌患者门静脉血CK20mRNA、癌组织中CD44v6、PCNA表达阳性率明显高于良性病变对照组（P＜0.01）和正常对照组（P＜0.0

2、1）；大肠癌组织中CD44v6及PCNA的表达与门静脉中CK20mRNA的表达有显著相关性（γ1=0.933,γ2=0.906,P＜0.05）；肝转移组CK20mRNA、CD44v6、PCNA阳性表达率均高于非肝转移组（P＜0.05）。结论：联合检测CD44v6、PCNA及CK20mRNA预测大肠癌肝转移，可显著提高预测的灵敏度及特异性，对于大肠癌早期肝转移监测具有很高的临床价值。【关键词】CK20mRNA・CD44v6・增殖细胞核抗原・结肠肿瘤・直肠肿瘤・肿

3、瘤转移・肝StudyonthepredictionofearlylivermetastasisofcolorectaltumorbydetectionofCK20mRNA、CD44v6andPCNA11【ABSTRACT】Objective:Toobtainsomeeffectiveobjectivemarkersusedtopredicttheearlylivermetastasisofcolorectaltumor,therelationshipoflivermetastasisofcolorectaltumo

4、rwithassociatedetectionthreemarkerssuchasCK20mRNA、CD44v6andPCNAwasstudied.Methods:TheexpressionofCK20mRNAinportalvenousbloodfrom30colorectalcancerpatientswasdetectedbyfluorescentquartoRTPCR,andtheresultsofCD44v6andPCNAincolorectalcancertissueweredeterminedbymeansofim

5、munohistochemistry,andthencomparedwithcontrolgroupsthroughstatisticsanalysis.Results:TherateofpositiveexpressionofCK20mRNAincolorectalcancerpatients’portalvenousbloodwasobviouslysuperiortothelevelofbenignpathologicalchangescontrols(P＜0.01),andsignificantlyhigherthant

6、hatofnormalcontrols(P＜0.01).TherateofpositiveexpressionofCD44v6andPCNAincolorectaltumortissuewasdistinctlysuperiortothelevelofbenignpathologicalcontrols,andremarkablehigherthanthatofnormalcontrols(P＜0.01).Thepositiveexpressionoflivermetastasiswasalsoclearlyhigherthan

7、thatofnolivermetastasis(P＜0.05).TherateofpositiveexpressionofCK20mRNAinportalvenousbloodwasevidentlycorrelatedtotheexpressionof11CD44v6andPCNAintumortissue(γ1=0.933,γ2=0.906,P＜0.05).TheresultsofassociatedetectionofCK20mRNA、CD44v6andPCNA6werecloselyrelatedtotheinciden

8、ceoflivermetastasis.Conclusion:IfcombinedthesemarkersofCK20mRNA、CD44v6andPCNAtoforecastlivermetastasisofcolorectaltumor,thesensitiv