姜黄素对单侧输尿管梗阻后肾间质纤维化的调节

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1、姜黄素对单侧输尿管梗阻后肾间质纤维化的调节作者：张彬王全胜朱锐汪永辉张丽晓徐敏刘建国【摘要】目的：研究姜黄素在单侧输尿管梗阻(UUO)大鼠模型中能否减少肾间质中转化生长因子β1（transforminggrowthfactor-β1,TGF-β1）和结缔组织生长因子（connectivetissuegrowthfactor，CTGF）的表达。方法:将30只大鼠随机分为3组，每组10只：假手术组、模型组、姜黄素组。模型组和姜黄素组行右侧输尿管接扎术，假手术组只游离不接扎。术后第14天处死各组中的大鼠，检测血液尿素

2、氮（BUN）、血清肌酸酐（Scr）。用免疫组织化学方法测定TGF-β1、CTGF的表达情况，同时采用Masson染色，观察肾脏病理情况。结果:姜黄素降低了BUN、Scr的含量，同时姜黄素显著减少了大鼠肾间质TGF-β1、CTGF的表达，并有效改善了肾脏的病理学损伤。结论:姜黄素可以减少输尿管梗阻后TGF-β1、CTGF的表达，从而改善UUO所致的肾脏损伤，提示姜黄素可能有延缓慢性肾脏疾病纤维化进程的潜能。【关键词】输尿管梗阻；纤维化；姜黄素；免疫组织化学；大鼠，Spragua-Dawley；肾间质［Abstra

3、ct］Objective:Toverifyifcurcumincandecreasethe5expressionoftransforminggrowthfactor-β1(TGF-β1)andconnectivetissuegrowthfactor(CTGF)inunilateralureteralobstruction(UUO)ratmodelsduringtheprocessofrenalinterstitialfibrosiscausedbyunilateralureteralobstruction(UU

4、O).Methods:TheexperimentwassuccessfullyconductedfromMaytoAugust,2007inUnionHospitalAffiliatedtoTongjiMedicalCollegeofHuazhongUniversityofScienceandTechnology.ThirtySprague-Dawleyratswererandomlydividedintothreegroups:shamoperatedgroup，UUOmodelgroup,andcurcum

5、in-treatedgroup(10ineachgroup).TheratsintheUUOmodelgroupandcurcumin-treatedgroupunderwentUUOsurgery.Ratsincurcumin-treatedgroupweregivenwatersuspensionofcurcuminpowder(200mg·kg-1·d-1)orally,whilethoseinUUOmodelgroupandshamoperationgroupweregivenwateronlyinst

6、ead.Alloftheratswerekilledin14daysaftersurgery.Thelevelsofbloodureanitrogen(BUN)andserumcreatinine(Scr)weremeasured,histologicalchangeswereobservedwithMassonstainmethod,andImmunohistochemistrywasperformedonrenaltissuetodetectTGF-β1andCTGF.Results:Intheobstru

7、ctedkidneys，thereweresignificantrenalinterstieialfibrosisandinterstitialspaceexpansion.ImmunohistochemicalstainingshowedthattheexpressionofTGFβ1andCTGFwassignificantly5increasedintheobstructedkidneys.Incomparisonwiththemodelgroup，BUNandScrlevels,interstitial

8、volume,andtheexpressionofTGF-β1andCTGFincurcumin-treatedgroupweresignificantlydecreased.Conclusions:Theresultsindicatethatcurcumincandown-regulatetheexpressionofTGF-β1andCTGFinUUOrats,throughwhi