哇巴因对大鼠肾皮质na+k+atp酶活性及多巴胺d1受体mrna表达的影响

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1、哇巴因对大鼠肾皮质Na+K+ATP酶活性及多巴胺D1受体mRNA表达的影响作者：张玉蓉，袁祖贻，任延平【摘要】目的观察哇巴因对大鼠肾皮质Na+K+ATP酶活性及多巴胺D1受体mRNA表达的影响。方法28只SD大鼠随机分为2组，即哇巴因组[27.8μg/(kg·d)哇巴因腹腔注射]和对照组(生理盐水1mL/d腹腔注射)，每周测量鼠尾动脉血压，共5周。于第3、5周后分2批处死动物，应用分光光度法测定各组大鼠肾皮质Na+K+ATP酶活性，同时采用实时定量PCR(realtimePCR)观察肾皮质中多巴胺D1受体mRNA

2、表达的变化。结果大鼠腹腔注射哇巴因3周后，两组血压无显著性差异，但哇巴因组大鼠肾皮质Na+K+ATP酶活性高于对照组(P<0.01)，多巴胺D1受体mRNA表达低于对照组(P<0.01)。5周后，哇巴因组大鼠血压与对照组相比有显著性差异(P<0.01)，哇巴因组大鼠肾皮质Na+K+ATP酶活性进一步增高(P<0.01)，多巴胺D1受体mRNA表达进一步减低(P<0.01)。结论长期小剂量外周注射哇巴因可使SD大鼠血压持续升高，这一作用与肾近曲小管Na+K+ATP酶活性增加引起水钠潴留有

3、关，多巴胺D1受体可能参与了这一过程。【关键词】Na+K+ATP酶活性;多巴胺D1受体;哇巴因;高血压;钠转运13　　MedicalSchoolofXianJiaotongUniversity,Xian710061,China)ABSTRACT:ObjectiveTostudytheeffectsofchronicouabaintreatmentonNa+K+ATPaseactivityandtheexpressionofdopamineD1receptorinratkidneycortex.MethodsAtot

4、alof28maleSpragueDawley(SD)ratswererandomlydividedintoouabaingroupandcontrolgroup,whichweretreatedwithouabainorsalinefor5weeks;rattailsystolicbloodpressure(SBP)wasrecordedweekly.Ratsweresacrificedafter3and5weeks,respectively.ThenNa+K+ATPaseactivityandtheexpressio

5、nofdopamineD1receptorinratkidneycortexweremeasuredbycolorimetricassayandrealtimePCR,respectively.ResultsAfter3weeksofouabaintreatment,themeanSBPdidnotchangesignificantly,buttheNa+K+ATPaseactivityincreased(P<0.01)andtheexpressionofdopamineD1receptordecreased(P&

6、lt;0.01)incomparisonwiththoseincontrolgroup.After5weeks,themeanSBPwassignificantlyhigherinouabaingroupthanincontrolgroup(P<0.01);theNa+K+ATPaseactivityfurtherincreased(P<0.01)andtheexpressionofdopamineD1receptorfurtherdecreased(P<0.01).ConclusionChronicpe

7、ripheraladministrationoflowdoseouabaincanconstantlyraisebloodpressureofrats,whichmayberelated13totheincreasedrenalsodiumretentioninducedbytheincreasedrenalcorticalNa+K+ATPaseactivity.DopamineD1receptormightbeinvolvedinthisprocess.　　KEYWORDS:Na+K+ATPaseactivity;

8、dopamineD1receptor;ouabain;hypertension;sodiumhandling　　肾脏是人体负责钠排泄的重要器官，其对水钠排泄的异常是高血压发病机制中的重要环节，其中肾脏近曲小管钠水重吸收增加是高血压形成的主要机制[1]。肾脏近曲小管上皮离子转运