肾衰2号方对慢性肾衰大鼠肾皮质环氧化酶2及环氧化酶1mrna表达的影响

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1、肾衰2号方对慢性肾衰大鼠肾皮质环氧化酶2及环氧化酶1mRNA表达的影响：蒲冠军,王琛,郑平东,何立群【摘要】目的：观察肾衰2号方对慢性肾衰（chronicrenalfailure，CRF）大鼠肾皮质环氧化酶2（cyclooxygenase2，COX2）及环氧化酶1（cyclooxygenase1，COX1）mRNA表达的影响。方法：采用左肾动脉的2/3分支结扎，右肾摘除（ablation/infarction,A/I）法制作大鼠CRF模型。造模成功后，将造模大鼠随机分为模型组、西乐葆（塞来昔布）组、肾衰2号方组。选用10只正常大鼠

2、作为正常对照组。治疗2个月后，苏木精和伊红染色观察大鼠肾组织形态学改变，检测治疗前后大鼠血清肌酐（serumcreatinine，SCr）、尿素氮（bloodureanitrogen，BUN）水平，并用逆转录实时聚合酶链反应检测肾组织中COX2和COX1mRNA的表达。结果：肾衰2号方能明显降低CRF大鼠SCr和BUN水平，改善肾功能及肾组织形态，并能明显降低CRF大鼠肾皮质COX2mRNA表达量，但对COX1mRNA表达无明显影响。结论：肾衰2号方可通过抑制COX2mRNA的过度表达改善肾功能，减轻肾小球硬化和纤维化。【关键词

3、】慢性肾功能衰竭;环氧化酶2;肾小球硬化;肾脏纤维化;大鼠Cyclooxygenase2(COX2)andcyclooxygenase1(COX1)aretheratelimitingenzymesinsynthesizingprostaglandins(PGs).Accordingtostudiesabroad,COX2metabolitesimplicatedinresidualglomerulardamage［1］andabnormalPGsofCOX2involveinchangesofglomerularhemod

4、ynamics,eextent,haveconnectionsentofglomerularsclerosisandthedeteriorationofrenalfunction［2］.Therefore,inhibitingoverexpressionofCOX2maybeoneoftheeffectiveetabolitesplayimportantrolesinmanyaspectssuchaskidneydevelopmentandregulationofrenalhemodynamics.Inrecentyears,than

5、kstothecontinuouseffortsofexplorationandpractice,traditionalChinesemedicinehasachievedpreliminaryresultsinpreventingandtreatingCRF.Basedonthepreviousresearch,echanismfromthepointofvieistry,pathologyandmolecularbiology.　　1Materialsandmethods　　1.1Experimentalmaterials　　1.1.

6、1ExperimentalanimalFiftySDmalerats,SPF,p;KLaboratoryAnimalCorp.Ltd.TheanimalcertificationnumberisSCXK(Hu)20030002.　　1.1.2DrugsandreagentsTheNo.2RFRconsistsofDangshen(RadixCodonopsis)15g,Yinyanghuo(HerbaEpimediiBrevicornus)15g,Danshen(RadixSalviaeMiltiorrhizae)15g,prepare

7、dDahuang(RadixetRhizomaRhei)15g,Zisu(PerillafrutescensL.)15g,Huanglian(RhizomaCoptidis)15g,Dongchongxiacao(Cordyceps)hyphae5g,Taoren(SemenPersicae)15gandChuanxiong(RhizomaChuanxiong)15g,adeintodecoctionL.Celebrex(alsocelecoxib,batchnumberJ20030099,100mg/tablet)aceuticalCo

8、.,Ltd.Celebrexg/mL.TRIzol,MMuLV(200U/μL)andRNAsin(40U/L)reagentsInvitrogenCorporation.DNAmarker