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ID:15151361
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页数:13页
时间:2018-08-01
《咪唑安定对豚鼠噪声性聋的保护作用》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、咪唑安定对豚鼠噪声性聋的保护作用作者:温健,景桂霞,高艳凤,王伟,许珉【摘要】目的通过检测豚鼠血清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和耳蜗中活性氧含量研究咪唑安定对噪声性聋的保护作用。方法雄性杂色豚鼠40只,随机分为正常对照组(C组)、咪唑安定组(M组)、生理盐水组(S组)和噪声性聋组(D组),每组10只。M组、S组和D组每天接触倍频程连续噪声(中心频率为4kHz,强度为100dBSPL)3h,连续3d。M组于噪声暴露前24h、即刻及噪声暴露中肌肉注射咪唑安定0.1mg/kg;S组在相同时间肌肉注射等
2、量的生理盐水;D组为单纯接触噪声对照组。C组不接触噪声仅肌肉注射咪唑安定,注射时间及剂量与M组相同。所有动物均于噪声暴露前及暴露后立即检测听性脑干反应(ABR)阈值,在噪声暴露前和噪声暴露第3天处死前,取血测定血清中SOD活性、MDA含量,于第3天测完ABR阈值后,立即断头处死豚鼠,取出双侧耳蜗,测定其活性氧含量。结果噪声暴露后,M组的ABR阈移(1.6±1.5)及活性氧含量[(291.10±2.30)u/mL]显著低于S组和D组[41.7±3.3,44.3±3.9;(348.52±3.60)u/mL,(315.56±
3、6.70)u/mL,P<0.05]。M组在噪声暴露后血清SOD活性下降、MDA含量升高,但其幅度明显小于S组和D组。结论咪唑安定可减少噪声暴露后耳蜗中增多的活性氧含量,从而产生对噪声性耳蜗损伤的保护作用。13【关键词】耳蜗;活性氧;噪声性聋;咪唑安定ChinaABSTRACT:ObjectiveToinvestigatetheprotectiveeffectsofmidazolamonnoise-inducedhearinglossinguineapigsbytestingreactiveoxygenspecie
4、s(ROS)levelinthecochleaandplasmaSODandMDA.MethodsTotallyfortymalepigmentedguineapigswererandomlydividedintofourgroups:control(C),midazolam(M),normalsaline(S)andnoise-induceddeafened(D)groups,withtenguineapigsineach.GroupsM,SandDwereexposedtoacontinuousnoise(4kHz
5、,octaveband,100dBSPL)3heverydayfor3consecutivedays.GroupMwastreatedwithmidazolam,whichwasadministeredintramuscularly(0.1mg/kg)24hbeforenoiseexposure,andimmediatelyuponandduringnoiseexposure.GroupSwasexposedtonoiseandtreatedwiththesamevolumeofnormalsalineintramus
6、cularly,thetimeofinjectionwasthesameasthatofGroupM.GroupCwasnotexposedtonoise,butwastreatedwithmidazolamintramuscularly,thetimeofinjectionandthedosagewerethesameasthoseofGroupM.GroupSwasexposedtonoiseandtreatedwithnormalsalineintramuscularly,thetimeofinjectionwa
7、ssamewiththatofGroupM.GroupDwasexposedtonoiseonly.Allanimalsreceivedauditorybrainstemresponse(ABR)thresholdrecording13beforeandimmediatelyafternoiseexposure.BloodwascollectedwhentheguineapigswerekilledafterthelastABRthresholdrecording,andserumSODactivityandMDAco
8、ntentweredetected.BoththecochleaewereremovedandpreparedforROSassay.ResultsAfternoiseexposure,ABRthresholdshift(1.6±1.5)andROScontent[(291.10±2.30)u/mL]inGroupMweresig
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