咪唑安定对豚鼠噪声性聋的保护作用

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1、咪唑安定对豚鼠噪声性聋的保护作用作者：温健，景桂霞，高艳凤，王伟，许珉【摘要】目的通过检测豚鼠血清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和耳蜗中活性氧含量研究咪唑安定对噪声性聋的保护作用。方法雄性杂色豚鼠40只，随机分为正常对照组(C组)、咪唑安定组(M组)、生理盐水组(S组)和噪声性聋组(D组)，每组10只。M组、S组和D组每天接触倍频程连续噪声(中心频率为4kHz，强度为100dBSPL)3h，连续3d。M组于噪声暴露前24h、即刻及噪声暴露中肌肉注射咪唑安定0.1mg/kg；S组在相同时间肌肉注射等

2、量的生理盐水；D组为单纯接触噪声对照组。C组不接触噪声仅肌肉注射咪唑安定，注射时间及剂量与M组相同。所有动物均于噪声暴露前及暴露后立即检测听性脑干反应(ABR)阈值，在噪声暴露前和噪声暴露第3天处死前，取血测定血清中SOD活性、MDA含量，于第3天测完ABR阈值后，立即断头处死豚鼠，取出双侧耳蜗，测定其活性氧含量。结果噪声暴露后，M组的ABR阈移(1.6±1.5)及活性氧含量[(291.10±2.30)u/mL]显著低于S组和D组[41.7±3.3，44.3±3.9；(348.52±3.60)u/mL，(315.56±

3、6.70)u/mL，P<0.05]。M组在噪声暴露后血清SOD活性下降、MDA含量升高，但其幅度明显小于S组和D组。结论咪唑安定可减少噪声暴露后耳蜗中增多的活性氧含量，从而产生对噪声性耳蜗损伤的保护作用。13【关键词】耳蜗；活性氧；噪声性聋；咪唑安定ChinaABSTRACT:ObjectiveToinvestigatetheprotectiveeffectsofmidazolamonnoise-inducedhearinglossinguineapigsbytestingreactiveoxygenspecie

4、s(ROS)levelinthecochleaandplasmaSODandMDA.MethodsTotallyfortymalepigmentedguineapigswererandomlydividedintofourgroups:control(C),midazolam(M),normalsaline(S)andnoise-induceddeafened(D)groups,withtenguineapigsineach.GroupsM,SandDwereexposedtoacontinuousnoise(4kHz

5、,octaveband,100dBSPL)3heverydayfor3consecutivedays.GroupMwastreatedwithmidazolam,whichwasadministeredintramuscularly(0.1mg/kg)24hbeforenoiseexposure,andimmediatelyuponandduringnoiseexposure.GroupSwasexposedtonoiseandtreatedwiththesamevolumeofnormalsalineintramus

6、cularly,thetimeofinjectionwasthesameasthatofGroupM.GroupCwasnotexposedtonoise,butwastreatedwithmidazolamintramuscularly,thetimeofinjectionandthedosagewerethesameasthoseofGroupM.GroupSwasexposedtonoiseandtreatedwithnormalsalineintramuscularly,thetimeofinjectionwa

7、ssamewiththatofGroupM.GroupDwasexposedtonoiseonly.Allanimalsreceivedauditorybrainstemresponse(ABR)thresholdrecording13beforeandimmediatelyafternoiseexposure.BloodwascollectedwhentheguineapigswerekilledafterthelastABRthresholdrecording,andserumSODactivityandMDAco

8、ntentweredetected.BoththecochleaewereremovedandpreparedforROSassay.ResultsAfternoiseexposure,ABRthresholdshift(1.6±1.5)andROScontent[(291.10±2.30)u/mL]inGroupMweresig