单侧睾丸扭转复位后对侧睾丸组织cgrp和ldh的测定

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1、单侧睾丸扭转复位后对侧睾丸组织CGRP和LDH的测定【摘要】目的?：了解降钙基因相关肽(calcitoningene-relatedpeptide，CGRP)浓度在单侧睾丸扭转复位后对侧睾丸组织中不同时段的变化，阐明CGRP与对侧睾丸早期损伤之间的联系。方法：将30只青春期前SD大鼠随机分为假手术组、扭转复位组和扭转切除(不复位)组，每组10只。假手术组作为对照;扭转复位组和扭转切除组依Turner法建立左侧睾丸扭转模型，在扭转4h后，前者行复位固定术，后者行切除术。三组分别于实验完成后4h、24h各处死大

2、鼠5只，取右侧睾丸组织行CGRP及乳酸脱氢酶(actatedehydrojgenase，LDH)浓度检测。结果：扭转切除组4h与24h时CGRP浓度分别为(712.4±23.1)μmol/L与(733.0±30.0)μmol/L，LDH浓度分别为(417.2±25.9)U/L与(458.1±23.5)U/L;而扭转复位组的CGRP在4h与24h时分别为(852.3±33.9)μmol/L与(861.7±29.8)μmol/L，LDH分别为(690.0±28.8)U/L与(706.7±32.3)U/L;显示C

3、GRP与LDH浓度在扭转复位组均有不同程度升高(P?<0.05)，但各指标在扭转切除组与假手术组的相比中未显示明显的统计学差异。结论：在单侧睾丸扭转复位后，对侧睾丸组织可能存在缺氧状态，CGRP的反应性升高可能起一定保护作用。【关键词】单侧睾丸扭转;睾丸损伤;降钙基因相关肽;大鼠8　Abstract:?Objective:TounderstandthechangesofCGRPconcentrationoncontralateraltestisofSDratswithunilateraltesticu

4、lartorsionindifferenttimeintervals，andexplaintheassociationbetweenCGRPandtheinjuryoncontralateraltestis.Methods:?ThirtyprepubertalSprague-Dawleyratswererandomlydividedinto3groups，?(10ratsineachgroup).Theratsofshamoperationgroupunderwentashamoperationofthel

5、efttestisundergeneralanaesthesia.Thoseoftorsion/detorsiongroupandthetorsion/orchiectomygroupunderwentasimilaroperationbutthelefttestiswasrotated720°clockwiselyfor4h，maintainedbyfixingthetestestothescrotum，thendetorsionwasperformedinthetorsion/detorsiongrou

6、p，whileorchiectomywasperformedinthetorsion/orchiectomygroup.Therighttestesoffiveratsfromeachgroupwereremovedandcollectedat4hourand24hourintervalsrespectivelyaftersurgery.Thenthebiochemicalparameters，suchascalcitoningenerelatedpeptide(CGRP)?andlactatedehydr

7、ogenase?(LDH)?wereevaluated.Results:?Intorsion/orchiectomygroup，theCGRPconcentrationsat4hand24hintervalswere?(712.4±23.1)μmol/Land(733.0±30.0)μmol/L，theLDHconcentrationswere(417.2±25.9)U/Land(458.1±23.5)U/L?;whileintorsion/detorsiongrouptheCGRPconcentratio

8、nswere(852.3±33.9)μmol/Land(861.7±29.8)μ8mol/L，theLDHconcentrationswere(690.0±28.8)U/Land(706.7±32.3)U/L;whichshowedthebiochemicalparametersinthetorsion/detorsiongroupincreasedindifferentdegrees?(P?<0.05).