-nmma对大鼠单侧睾丸扭转后对侧睾丸生精功能的保护作用

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1、-NMMA对大鼠单侧睾丸扭转后对侧睾丸生精功能的保护作用【摘要】目的:探讨N-甲基-L-精氨酸（L-NMMA）对大鼠单侧睾丸扭转后对侧睾丸生精功能的保护作用。方法:42只健康雄性SD大鼠随机均分为假手术组、安慰剂组和L-NMMA组。建立左侧睾丸扭转模型。安慰剂组和L-NMMA组大鼠分别于扭转复位前30min通过尾静脉缓慢推注生理盐水和L-NMMA。三组大鼠分别于术后1周、2个月时处死7只，即刻采集各组大鼠未扭转侧睾丸标本，测定睾丸组织丙二醛(MDA)水平，同时进行组织病理学观察。结果:与安慰剂组相比，L-NMMA组1周时MDA值明显下降，平均曲细精管直径明显升高，而睾丸结构评分则明

2、显偏低（P＜0.05）;2个月时主要表现在生精上皮层数和平均曲细精管直径较高，睾丸结构评分较低，两组间比较均有统计学差异（P＜0.05）。结论:L-NMMA可以减轻大鼠单侧睾丸扭转后对侧睾丸的早期损害，对晚期对侧睾丸的生精功能具有一定的保护作用。【关键词】L-NMMA睾丸扭转生精功能Abstract:Objective:Toexplorethebiochemicalandhistologicalchangesinthecontralateraltestisofratswithunilateraltesticulartorsionandelucidatetheprotectiveef

3、fectsofL-NMMAonthecontralateraltesticular9spermatogeneticfunction.Methods:Fouty-twoprepubertalSprague-Dawleyratswererandomlydividedinto3groups,with14ratsineach.Theratsinsham-operatedgroupunderwentashamoperationofthelefttestisundergeneralanaesthesia.TheratsinsalinegroupandL-NMMAgroupunderwentas

4、imilaroperationbutthelefttestiswasrotated720°clockwisefor4h,maintainedbyfixingthetestistothescrotum,andsaline/L-NMMAinfusedduringhalfanhourbeforedetorsionwasperformed.Alltheright(untwisted)testiswereremovedfromratsat1weekand2monthsintervalsaftersurgery,7ratsweretakenfromeachgroupeachtime.Thent

5、hemalondialdehyde(MDA)levelswereevaluated;histologyandseminiferoustubulediameterswereassessed.Results:Comparedwithsalinegroup,theMDAlevelsinL-NMMAgroupdecreasedsignificantlywhilethemeanseminiferoustubulardiameterofcontralateraltestisandhistologicalgradesmaintainedinabetterconditionat1weekinter

6、val;anditwasalsoknownthattheratsinL-NMMAgroupgotahigherscoreatthegradesofgermcelllayersandmeanseminiferoustubulardiameterofcontralateraltestisat2monthsinterval.Conclusion:L-NMMAcanreducethedamageofcontralateraltestisofprepubertalratswithunilateraltesticulartorsionduringtheearlyperiod,andL-NMMA

7、mayalso9haveaprotectiveeffectonthetesticularspermatogeneticfunctioninthelaterstage.Keywords:L-NMMA;testiculartorsion;testicularspermatogeneticfunction睾丸扭转是常见的泌尿外科急症，多见于25岁以下青少年,发病率约为1/4000，即使及时手术复位固定，仍可能引起对侧睾丸生精功能的损伤[1]。单侧睾丸扭转后如何保护对侧睾丸的