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1、健脾与补肾对脾虚模型大鼠学习记忆及脑内P物质、血管活性肠肽的影响【摘要】目的探讨脾虚模型大鼠学习记忆和脑内P物质(SP)、血管活性肠肽(VIP)的变化,以及健脾、补肾中药对上述变化的改善作用。方法采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,检测健脾与补肾对其学习记忆及脑内SP、VIP变化的影响。结果水迷宫实验学习期和记忆期,模型组游全程时间长,错误次数多；健脾组与补肾组游全程时间短,错误次数少。跳台实验学习期和记忆期,模型组错误次数较多,潜伏期短；健脾组错误次数较少,潜伏期长；在学习期补肾组错误次数较少,潜伏期较长,但记忆期错误次
2、数较多。模型组SP免疫阳性反应物在下丘脑腹侧核、海马CA1区、前额叶皮层显著减少；健脾组在上述脑区则显著增加；补肾组海马CA1区和前额叶皮层明显增加,但在下丘脑腹侧核减少。模型组VIP免疫阳性反应物在海马CA1区、前额叶皮层显著减少；健脾组与补肾组海马CA1区、前额叶皮层VIP免疫阳性反应物显著增加。结论脾虚模型大鼠学习记忆及脑内对学习记忆有促进和增强作用的SP、VIP明显下降。健脾、补肾中药对上述变化有明显改善作用。【关键词】脾虚模型大鼠；学习记忆；P物质；血管活性肠肽Abstract：ObjectiveToinquireintothe
3、changesoflearningandmemoryabilityandthelevelofSP,VIPinthebrainofthemodel11ofSpleeninsufficiency.Toobservetheinfluenceinvigoratingspleenandrestoringvirilityonabovechanges.MethodsEstablishingthespleeninsufficiencymodelofratbyadoptingbitterdeclinediarrhea,wildeatingandoverwo
4、rking,toexamineinfluenceofinvigoratingspleenandrestoringvirilityontheabilityoflearningandmemorywiththechangeofSPandVIPinthebrain.ResultsIntheperiodofstudyandrememberofthewatermazeexperiment,theswimwholedistancetimewaslongandmistakenumberwasmanyinthemodelgroup；theswimwhole
5、distancetimewasshortandthemistakenumberwaslittleinthegroupofinvigoratingtheSpleenandrestoringvirility.Intheperiodofstudyandrememberofthedivingplatformexperiment,themistakenumberoftimesismoreandtheincubationperiodwasshortinthemodelgroup；themistakenumberwaslessandtheincubat
6、ionperiodwaslonginSpleeninvigratinggroup.Attheperiodofremembermistakenumberwasmore.Inthemodelgroup,theimmunoreactionmasculinesubstanceofSPleveldecreaseobviouslyinthehypothalamusventralkernel,thehippocampiCA1areaandforehandcortex；onabovementionedparts,thegroupofinvigoratin
7、gspleenandrestoringvirilityincreasesdistinctly,inthegroupofrestoringvirilityincreasesdistinctlyinthehippocampiCA1areaandforehead11cortex,butdecreasedinhypothalamusventralkernel.Inthemodelgroup,theimmunoreactionmasculinesubstanceofVIPleveldecreasedobviously；inthehippocampi
8、CA1areaandcortex；onabovementionedparts,thegroupsofinvigoratingspleenandrestoringvirilityincrease
