乳猪肝胶原蛋白水解物注射液的研制及对荷瘤小鼠肿瘤生长的影响

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1、乳猪肝胶原蛋白水解物注射液的研制及对荷瘤小鼠肿瘤生长的影响作者：邹晓军廖汉雄王耐勤杨联萍，刘彤，邹清雁【摘要】目的与方法摸索乳猪肝胶原蛋白水解物注射液（HCH）的制备方法,同时对其进行质量检验和初步稳定性试验；并观察其对荷瘤昆明小鼠HepA肝瘤和S180纤维肉瘤及小鼠体内肝癌H22移植瘤的影响。结果HCH生产工艺路线合理，质量达到拟定标准要求。HCH能明显抑制HepA肝瘤生长，25、50、100、200mg·kg-1·d-1的抑制率分别为43.26％、55.06％、74.71％、40.47％；对荷瘤小鼠S180纤维肉瘤25、50

2、、100mg·kg-1·d-1的抑制率分别为53.87％、76.53％、58.20％，而阿霉素（2mg·kg-1·d-1）阳性对照组的抑制率为62.41％；对小鼠体内肝癌H22移植瘤生长的抑制呈剂量相关性，50、100、200mg·kg-1·d-1的抑制率分别为48.1％、60.0％、74.8％，而卡铂（20mg·kg-1·d-1）阳性对照组的抑制率为63.7％。结论此法可制备合格的HCH，且适合于大规模生产；HCH能明显地抑制荷瘤小鼠肿瘤的生长。【关键词】胶原；肿瘤；生长抑制；小鼠　　Abstract:ObjectiveToi

3、nvestigatethemethodstopreparethehepaticcollagenoushydrolysates(HCH)forinjection,11therudimentstabilitytest,andthequalitycontrolstudyoftheHCH,andtoobserveitseffectonthetumorgrowthinthetumorbearingmice.MethodsThedissolvabilitycollagenwereextractedfromthesucklingpiglive

4、rwiththebiochemicalapproach,thetumorinhibitingpolypeptidesforinjectionwerepreparedwiththepepsinhydrolysis.Thequalitycriteriaoftheproductwerestudied.TheeffectsofTIPontheHepAhepatoma,H22hepatomaandS180fibrosarcomawereinvestigated.ResultsTheproductsqualityreachedthequa

5、litycriteriashowedthattheproductionprocedureofTIPwassuitable.TheresultsshowedthatTIPcouldapparentlyinhibitthegrowthofHepAhepatomainthetumor-bearingmicewiththeinhibitoryratesof43.26％,55.06％,74.71％,40.47％atthedosesof25,50,100,200mg·kg-1·d-1TIP;andinhibitthegrowthofS180

6、fibrosarcomawiththeinhibitoryratesof53.87％,76.53％,58.20％attherespectivedosesof50,100,and200mg·kg-1·d-1TIP,therelativeinhibitoryrateofthepositiveadriamycin（2mg·kg-1·d-1）controlwas62.41%;TIPcouldapparentlyinhibitthegrowthofH22hepatomatransplantedinthemiceatadose-depend

7、antwaywiththeinhibitoryratesof48.1％,60.0％,74.8％attherespectivedosesof50,100,and200mg·kg-1·d-1HCH,andtherelativeinhibitoryrateofthepositive11adriamycin（20mg·kg-1·d-1）controlwas63.7%.ConclusionHCHpreparedwiththebiochemicalapproachwasqualifiedandsuitableforlargescalepr

8、odiction.HCHcouldapparentlyinhibitthegrowthoftumortransplantedinthemice.　　Keywords:collagen;tumor;growthinhibition;mice　　O’Reilly等［