帕金森病大鼠模型多巴胺转运蛋白125i-β-cit放射自显影研究

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1、帕金森病大鼠模型多巴胺转运蛋白125I-β-CIT放射自显影研究　　【摘要】目的探讨125I-β-CIT[甲基-3β-（4-碘苯基）托烷-2β-羧酸盐]多巴胺转运蛋白（DAT）显像技术的临床应用价值。方法应用6-羟基多巴胺（6-OHDA）建立黑质部分损毁及完全损毁偏侧帕金森病（PD）大鼠模型。静脉注射125I-β-CIT40μCi进行放射自显影。高效液相-电化学法检测纹状体多巴胺（DA）及其代谢产物含量。免疫组化酪氨酸羟化酶（TH）染色观察黑质及纹状体TH阳性细胞及纤维。结果黑质部分损毁及完全损毁偏侧模型损毁侧纹状体125I-β-CIT放射活性分别为2.67±0.25和0.

2、98±0.29，较未损毁侧分别降低18%和72%，DA含量分别降低39%和98%。TH染色可见损毁侧黑质及纹状体TH阳性细胞及纤维明显少于对侧。结论DAT的显像研究可能有助于PD早期诊断及病情监测。　　AutoradiographicstudyofdopaminetransporterinratmodelsofParkinsondiseasewith125I-β-CIT　　LIUZhenguoCHENShengdi，SUNWenshan，etal　　DepartmentofNeurology，RuijinHospital，ClinicalandResearchCenterf

3、orParkinsonDisease，ShanghaiSecondMedicalUniversity，Shanghai200025．China　　【Abstract】ObjectiveTostudythevalueofimagingfordopaminetransporter（DAT）with125I-β-CIT．MethodsTheratmodelsofhemiparkinsonismwitheitherpartialorcompletelesionswererenderedwith6-hydroxydopamine（6-OHDA）．Eachratwasinjectedi

4、ntravenouslywith125I-β-CITcontaining40μCi．Coronaltissuesectionswereimagedbyautoradiography．Thelevelsofdopamine（DA）anditsmetabolitesweremeasuredbyhighperformanceliquidchoromatographyandelectrochemicaldetection（HPLC-ECD）．Thetyrosinehydroxylase（TH）-positivecellsandfibresinthesubstantianigraan

5、dstriatumoftheratswereobservedwithimmunohistochemicalstaining．ResultsTheradioactivitiesinthelesionsofthestriatumofbothpartialandcompletehemiparkinsonianlesionsoftheratmodelswere2．67±0.25and0．98±0.29，respectively，andweresingificantlydecreasedby18%and72%，respectively，ascomparedwiththoseonthe

6、sidewithoutlesions．ThelevelsofDAinthelesionsofthestriatumofpartialandcompleteratmodelsweredecreasedby39%and98%，respectively．ThelossofTH-positivecellsandfibresinthesubstantianigraandstriatumwasfoundinthelesionsofthestriatumofbothpartialandcompletehemiparkinsonianratmodels．ConclusionTheimagi

7、ngstudyofDATmaybehelpfulfortheearlydiagnosisofParkinson′sdiseaseandforthemonitoringoftheprogressionofthisdisease．　　【Keywords】Carrierproteins；Parkinsondisease；　125I-β-CIT；Autoradiography　　帕金森病（Parkinsondisease，PD）病理基础是中脑黑质多巴胺（DA）能神经元及黑质纹状体通路变性死亡。中枢多巴胺转运蛋白（DAT）位