pparγ配体罗格列酮对人肝癌细胞系hepg自噬影响及其机制探讨

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1、MASTERDISSERTATIONTheEffectofPPARγligandRosiglitazoneonAutophagyinHumanHepatomaCellLineHepG2andtheExplorationofitsMechanismCandidate:ZhangYanMajor:PathologyandPathophysiologySupervisor:Prof.GuanYangTongjiMedicalCollegeHuazhongUniversityofScienceandTechnolo

2、gyWuhan，430030,ChinaDecember,2011独创性声明本人声明，我所呈交的学位论文是在导师指导下进行的研究工作及取得的研究成果。本人保证论文中数据的真实性，论文中不包括其他人已经发表或撰写过的研究成果，或使用过的材料。据我所知，相关他人的成果已在论文中特别加以标注和致谢；尽我所能，与我共事者对本研究所做贡献均已在论文中作了明确的说明。本人完全意识到本声明的法律结果由本人承担。学位论文作者签名日期：年月日学位论文版权使用授权书本学位论文作者完全了解华中科技大学有关保留、使用学位论文的

3、规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许学位论文被查阅和借阅；本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□，不保密□。在年解密后适用本授权书。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月目录摘要······························································

4、·····················1ABSTRACT············································································2主要中英文缩略词表·································································3前言········································································

5、···········4材料和方法·············································································5结讨果·················································································13论··········································································

6、·······17参考文献··············································································21附综致图·················································································25述···········································································

7、······28谢·················································································40华中科技大学硕士学位论文PPARγ配体罗格列酮对人肝癌细胞系HepG2自噬的影响及其机制探讨摘要目的：观察经不同浓度PPARγ配体罗格列酮（RSG）诱导后人肝癌细胞系HepG2自噬水平的变化及自噬特异性抑制剂3-MA对罗格列酮诱导的HepG2细胞自噬的影响，并探讨其作用机制。方法：体外培养HepG2细胞，将细胞分为对照组、不同

8、浓度罗格列酮处理组（2µmol/L、4µmol/L、8µmol/L、16µmol/L）和3-MA抑制组。应用免疫组织化学方法检测罗格列酮作用后HepG2细胞中自噬相关基因Beclin1的表达以及添加自噬抑制剂3-MA后Beclin1的表达。透射电镜观察HepG2细胞自噬体的超微结构。流式细胞术检测HepG2细胞增殖细胞相关核抗原Ki67的表达。结果：经不同浓度罗格列酮作用后，HepG2细胞Becli