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1、母种分离技术（Motherseedseparationtechnology）Innature,ediblefungidonotexistalone,butlivetogetherwithmanybacteria,radiationbacteria,moldsandsoon.Theso-calledseparationofbacteriaistoseparatetheseediblefungilivingwiththebacteria,andtoobtainpureandexcellentstrainsthroughcu
2、lture.Spawn,stock,seedandcultivatedspecies.Seedproductionproceduresrefertofigure13.(1)isolationandcultureofmotherspeciesSeparationofmotherediblefungus,canbedividedintosporeseparation,tissueisolationmethodandhyphaseparationetc..1.sporeisolationmethodSporeisolatio
3、nmethodisthemethodofculturingmyceliumbyusingsexualsporesorasexualsporesofediblefungi.Thisstrainhasstrongviability,buttherearedifferencesbetweenindividualspores,andnaturaldifferentiationphenomenonisserious,variabilityislarge,needtobefruitingtestinordertobeapplied
4、inproduction.(L)singlesporeisolation:themethodofobtainingonlyonesporepertimeorineachtesttubeandallowingittogerminateintomyceliumtoobtainpurestrains.Bysinglesporeisolatedfromthemyceliumofmushroomandmushroom,havestrongability,thismethodcanbeusedtoproducepurestrain
5、sisolated.Singlesporeisolationisseldomusedinproduction,andthetechnologyiscomplex.(2)multisporeisolation:amethodofobtainingapurestrainofediblefungibyinoculatingmanysporesonthesamemedium,allowingthemtogerminateandfreelymate.Specificmethodsofoperation,therearethefo
6、llowing:Amushroomsporeejectionmethod:individualrobust,roundshapedflower,nopests,fruitinguniform,highyield,strongadaptabilityofthemushroom,eightorninematurecutparts,stipegauzeorcotton,washwateranddrywithfilterpapersurfacesterilizedseveraltimeswithsterilewater.Int
7、heindoorinoculationboxorthekindofsterile,mushroomgillsdownwithwirehangingupsidedowninaglassfunnelbelowthefunnelinvertedcoverinaPetridishabovetheupperholestuffedcotton.PutinaPetridishcoveredwithenameldiscgauze,standingfor12to20hours,thesporeswillbescatteredintheg
8、illsinPetridish.Theformationofalayerofpowder(mushroomsporeprintverypalepurple,brownmushroom,mushroom,mushroom,letinousedodeswhitesporeprint).Asmallamountofsporesstain
