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1、1对缺血-复灌心肌保护的作用第36卷第6期2006年l2月温州医学院JournalofWenzhouMedicalCollegeVbI_36No.6Dec.2OO6ATP敏感性钾通道参与HO一1对缺血一复灌心肌保护的作用Il汪洋.,徐和靖,朱立.,沈岳良.,陈莹莹.,杜友爱(温州医学院,浙江温州3250351.病生教研室;2.生理教研室;3.浙江大学医学院生理学教研室,浙江杭州310006)[摘要]目的:研究线粒体ATP敏感性钾通道(mitoK)参与血红素氧化酶l(Ho-1)对抗心肌缺血-复灌损伤.方法:sD大鼠腹腔注射Ho.1的诱导剂hemin(50mg/k
2、g),抑制剂znPP(25g/kg)和mitoK^通道阻断剂5.}Ⅱ),24h后取离体心脏给予30min缺血和120min复灌.检测心室收缩功能,LDH,CK和心肌梗死面积.结果:①hemin诱导Ho-l活性增加可改善缺血-复灌心脏的收缩功能,缩小心肌梗死面积,而znPP可抑制其作用.②在腹腔注射hemin前给予mitoK^r通道阻断剂5-1tD(5mg/kg),与Hemin+IS组相比,心脏的收缩功能明显下降,心肌梗死面积增大,LDH和CK释放增加.而在hemin预处理后24h与30min缺血前给予5-}Ⅱ)灌流(100vmol/L)同样可阻断hemin诱导
3、的心肌保护作用.结论:hemin可诱导心肌Ho.1增加保护心肌缺血-复灌性损伤,而mitoKAw通道在hemin诱导的心肌保护作用中扮演了启动因子和终末效应器双重角色.[关键词]心肌;缺血;血红素氧化酶-l;线粒体ATP敏感性钾通道[中图分类号]R331.31[文献标识码]A[文章编号]looo一2l38(2oo6)o6一o5l6—04TheprotectiveeffectsofATP-sensitivepotassiumchannelparticipatinginactivationofhemeoxygenase1(HO-1)onischemia-reper
4、fusioninjuryinheartWANGYank',tie-jing.z鼬Li.eta1.1Del~rtmentofPathophysiology.WenzhouMedicalCollege.Wenzhou.325035圈■墨■Objecrive:Toexpl.reandevaluatetheprotectiveeffectsofchromosomeATP-sensitivepotassiumchannelparticipatingintheactivationofhemeoxygenassel(H&I)againsttheinjuryofisc
5、hemia-reperfusionintheheart.№thods:HO-1inducerheroin(50m~kg),inhibitorznPP(25ugag)andtheblockerofmitoKATPchannelⅧwereinjectedintraperi~meally.Theisola~theartwassubjectedt030minregionalischemiaand120minofreperfusion.Theconstrictivefunctionoftheventricles.LDH.CKandtheinfractedareawere
6、measured.Results:①TheincreasingoftheactivationofHO-Iinducedbyhemincouldimprovetheconstrictivefunctionoftheischemia-reperfusedheartandshortenedtheinfractedarea,butZnPPcouldinhibiteitsfmction;②ComparedwithHeroin+IS,mitoK—blocker5-皿(5m~/kg)givenbeforeintraperitonealinjectionofhonincoul
7、dobviouslydeclinethecardiacconstrictivefunction,increasethecardiacinfrac~d~greaandincreasethereleasingofLDHand.Afterpretreatmentofheminfor24hrs,thecardiacprotectioninducedbyheroincouldbesimilarlyblockedwhenⅧwasgivenbefore30minofischemia.Conclusion:Hemincanprotectagainstischemi-reper
8、fusioninjuryinthehe
