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1、9、MMP-2表达的影响中风与神经疾病杂志2006年2月第23卷第1期文章编号:1003—2754(2006)01—0075—04?75?凝血酶对大鼠脑内MMP一9,MMP一2表达的影响江汉秋,刘群,刘瑾,张昱摘要:目的探讨凝血酶对大鼠脑内MMP一9,MMP一2表达的影响.方法Wistar大鼠随机分为假手术组及实验组.实验组脑内注入凝血酶.在不同时间点采用干湿重法测脑水含量.免疫组化方法检测脑内MMP一9,MMP一2的表达.假手术组注入等量生理盐水.结果脑组织水含量在凝血酶注入后6h开始增加.3d达高峰.MMP一9阳性细胞在注射凝血酶后6h即开始表达增加,与对
2、照组相比差异显着(,)<O.01),3d达高峰.随后持续下降.阳性微血管数也有相似的变化趋势.MMP一2阳性细胞在注射凝血酶后1d才开始有少量表达.后持续增多,5d达高峰,随后有所下降.但14d仍有明显表达,与对照组相比差异显着(,)<O.01).阳性微血管数也有相似的变化趋势.结论凝血酶在脑出血后脑水肿及脑组织损伤中起了关键的作用,MMP9,MMP一2参与了急性期脑水肿,炎症反应等过程,MMP一2在损伤修复中可能有重要作用.关键词:凝血酶;脑水肿;MMP一9;MMP一2;脑出血中图分类号:R743.34文献标识码:AExpressionofMMP
3、一9andMMP一2inratsbrainafterinjectionthrombin,,,^4lⅣ(ttan—qiu.,J,Qmh,J,【,lin.eta1.(DepartmentoJNeurology,FirstHospitaloJ.lilinniversityChangchun130021.China)Abstract:ObjectiveTostudyexpressionofMMP一9andMMP2inratsbrainafterinjectionthrombin.MethodsWistarratsweredividedrandomlyinto2grou
4、ps.shamoperatedgroupandexperimentgroup.Theani—malsofexperimentgroupwereinjectedthrombininbrain.Attheduetimesomeratswerekilledandbraintissuewasremovedtomeasurethewatercontentbydry—wetweightmethod.TheotherswereimmunohistochemicallystainedtomeasureoftheexpressionofMMP一9andMMP一2.Theanim
5、alsofsham—operatedgroupwereinjectednormalsaline.ResultsThewatercontentofbrainincreasedat6hafterinjectionthrombin.andpeakedat3d.Comparedwithsham—operatedgroup,inexperimentgrouptheexpressionofMMP一9positivecellswereobservedat6hafterinjectionthrombin(P<O.01).andpeakedat3d.thendecreas
6、edgradually.ThenumberofMMP一9positivemicro—bloodvesselhadthesamechangetendencyasthepositivecells.AfewofMMP一2positivecellswereobserveduntilldinexperimentgroup.andpeakedat5days.thendecreasedgradually.Theincreasingtendencylastedto14d.Thenumberofpositivecellsat14dwassignificantlymorethan
7、shamgroup(,)<O.01).ThistendencywassimilartothenumberofMMP一2positivemicro—bloodvesse1.ConclusionThrombinplaysanimportantroleinintracerebralhemorrhageoncerebraledemaandtissueimpairment.MMP一9andMMP一2contributetobrainedemaandinflammatoryreactionintheacutephaseofICH,andMMP一2isperhapsi
8、mportantinrehabilit
