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1、基质金属蛋白酶组织抑制剂-1基因在卵巢肿瘤中的表达及突变的检测肿瘤2006年6月第26卷第6期TumorVoi.26,No.6,Jun.2006ClinicalResearch?临床研究基质金属蛋白酶组织抑制剂一1基因在卵巢肿瘤中的表达及突变的检测胡晓霞,李力孙,黎丹戎.,张玮,唐步坚.(1.广西自治区人民医院妇产科,南宁530021;2.广西医科大学附属肿瘤医院妇瘤科,南宁530021)[摘要]目的:研究基质金属蛋白酶组织抑制剂一1(tissueinhibitorsofmatreixmetalloproteinases,TIM
2、P一1)在卵巢肿瘤中的表达及突变情况.方法:RT—PCR方法检测48例卵巢癌,21例良性卵巢肿瘤及22例正常卵巢组织中TIMP一1mRNA的表达情况;利用人卵巢癌组织构建人TIMP一1真核表达质粒并测序;聚合酶链反应一单链构象多态性(PCR—SSCP)分析技术检测卵巢恶性肿瘤,良性肿瘤和正常卵巢组织中TIMP一1基因的突变.结果:TIMP1在各种卵巢组织中mRNA的表达并无差异;构建的真核表达质粒经测序含人TIMP一1全长cDNA,于第6外显子处发生错义突变;卵巢恶性肿瘤中TIMP一1基因的突变率为16.7.结论:卵巢恶性肿瘤组
3、织中有TIMP-1基因突变存在;构建的TIMP一1真核表达质粒,为下一步研究TIMP-1在卵巢癌侵袭转移中的作用打下了物质基础.[关键词]卵巢肿瘤;金属蛋白酶1组织抑制剂;DNA,重组;基因表达;突变,错义[中图分类号]R737.31;Q786[文献标识码]A[文章编号]1000—7431(2006)06—0547—04ExpressionoftissueinhibitorofmatrixmetallOprOteinase1anditsmutationinovariantumortissuesHUXiao—Xia.LILi",I
4、IDan—Rong.,ZHANGWei,TANGBu—JJan(1.DepartmentofGynecologyandObstetrics.People'sHospitalofGuangxiProvince.Nanning530021,China;2.DepartmentofGynecologyOncology,TumorHospitalAffiliatedtoGuangxiMedicialUniversity,Nanning530021,China)[-ABSTRACT]Objective:Toinvestigatetheex
5、pressionoftissueinhibitorofmatrixmetalloproteinase1(TIMP一1)anditsmuta—tioninovariantumortissue.Method:ReversedtranscriptasepolymerasechainreactionwasusedtoassesstheexpressionofTIMP一1mRNAin48caseswithmalignantovariantumor,21caseswithbenignovariantumorand22casesfornorm
6、alovariantissue.TIMP一1eukaryoticexpressionrecombinantplasmidwasconstructedandthesequencewasanalyzed.ThemutationofTIMP-1inovariantumortissueswasdetectedbypolymerasechainreaction-singlestrandconformationpolymorphism(PCR-SSCP)technology.Results:Therewasnosignificantdiff
7、erenceintheexpressionofTIMP一1mRNAbetweenovarianmalignanttumor,benigntumor,andnormalovariantissue.Sequenceanalysisshowedthatconstructedeukaryoticexpressionplasmidcontainedfull—lengthcDNAofhumanTIMP一1gene,whichhadamissensemutationinexon6.Thegenemutationratewas16.7inova
8、rianmalignanttumortissues.Conclusion:MutationofTIMP-1geneoccursinovarianmalignanttumor.TIMP-1eukaryoticexpressionrecombinantplasmid
