资源描述：
《高效液相色谱法测定蔬菜中8种氨基甲酸酯类农药残留》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、高效液相色谱法测定蔬菜中8种氨基甲酸酯类农药残留现代食品科技ModernFoodScienceandTechnology2011,Vo1.27,No.7高效液相色谱法测定蔬菜中8种氨基甲酸酯类农药残留邵金良,黎其万,刘宏程,佴注,兰珊珊,梅文泉,杜丽娟,樊建麟(云南省农业科学院质量标准与检测技术研究所,云南昆明650223)摘要:建立了固相萃取.高效液相色谱柱后衍生荧光检测蔬菜中涕灭威亚砜,涕灭威砜,灭多威,三羟基克百威,涕灭威,克百威,甲萘威及异丙威等8种氨基甲酸脂类农药残留的分析方法.本文对测定氨基甲酸酯农药
2、残留的分离条件,提取溶剂,洗脱溶剂,净化材料和基质效应进行了优化试验.结果显示,8种氨基甲酸脂类农药在0.1—1.0mg,L的浓度范围内线性良好,线性相关系数为0.99943--4).99992,检出限为0.008~0.01mg/kg之间.样品经乙腈提取,氨基小柱净化,采用基质加标,在0.1mgg添加水平的平均回收率为79.4119.0%,相对标准偏差2.5%～l1.5%;0.2mg/kg添加水平的平均回收率为81.2%～115.5%,相对标准偏差为2.8%8.1%.此方法可适用于大白菜,辣椒,茄子,番茄,菠菜,
3、苦瓜,结球甘蓝和豇豆等样品的氨基甲酸酯类农药残留的分析测定.关键词:高效液相色谱法;柱后衍生;氨基甲酸酯类农药;蔬菜文章篇号:1673.9078(2011)7.856.860Determination0fEightCarbamatePesticidesinVegetablesbyHighPLiquidChromatographyertormanceLiquitl11romato~rap11rSHAOJin-Liang,LIQi-Wan,LIUHong-cheng,ER-Zhu,LANShan-shanMEIWen
4、-quan,DULi-juan,FANJian-lin(InstituteofAgricultureQualitystandards&TestingTechnique,YunnanAcademyofAgriculturalScience,Kunming,Yunnan650223,China)Abstract:AnewmethodforthesimultaneousdeterminationofeightcarbamatepesticidesinChinesecabbage,pepper,egg-plant
5、,tomato,Chinesespinach,bittergourd,cabbageandcowpeabyhighperformanceliquidchromatographywasdeveloped.Eightcarbamatepesticideswasextractedfromthesamplesbyacetonitrile,salted,purifiedwithLC—NH2columnanddeterminedbyhighperformanceliquidchmmat0graphyusingpost-col
6、umnderivatizationwithfluorescencedetector.TheproposedmethodsshowedagoodlinearitybetweenanalyticalsignalintherangeofO.1～1.0mg,Lwiththelinearcorrelationcoefficientsof0.99943~0.99992andlimitsofdetectionof0.008-0.01mg/kg.Theaveragerecoveriesobtainedfromsamplesspi
7、kedwi幽standardsatconcentrationlevel0.1megkgand0.2mg/kgwereintherangeof79.4%-119.O%and81.2%一l15.5%,respectively,withrelativestandarddeviationsof2.5%～l1.5%and2.8%～8.1%,respectively.Themethodwasaccurate,reliable,andCanbeusedfordeterminationcarbamatepesticidesinv
8、egetables.Keywords:highperformanceliquidchromatography;post—columnderivatization;carbamatepesticides;vegetables氨基甲酸酯是一种具有良好杀虫效果,在我国使用量较大的广谱性农药【I】.该类农药是以甲酸酯为前体化合物发展而来的农药,具有分解快,残留期短,低毒,高效和选
