比色法测定苦瓜中的总皂甙

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1、比色法测定苦瓜中的总皂甙天然产物研究与开发NatProdR.esp20O6,18(Supp1):151—155文章编号:1001.6880(2006)Suppl-0151-05比色法测定苦瓜中的总皂甙石雪萍,姚惠源(江南大学食品学院,无锡214036)摘要:本试验通过对高氯酸和硫酸体系进行比较,选择香草醛.冰醋酸.高氯酸体系作为测定苦瓜皂甙的合适显色条件,并对体系中各个因素逐一考察,确定比色法测定苦瓜皂甙的条件为:0.2mL冰醋酸,0.8mL高氯酸,5%香草醛.冰醋酸溶液,70℃条件下反应20min.试验以苦瓜皂甙建立了

2、苦瓜皂甙测定回归曲线,线性和稳定性可靠,平均加标回收率100.78%,变异系数为1.910%.关键词:苦瓜;皂甙;比色法中图分类号:TS201.2;Q946.91文献标识码:ASpectrophotometricDeterminationofSaponinsfromMomordicacharantiaL.SHIXue—ping,YAOHui—yuan(SchoolofFoodSc/enceandTechno/ogy,SouthernYangtzeUn/vers/ty,Wuxi214036,China)Abstract:S

3、aponinsarcthemainactiveconsituentsintheMomordicachalxlllt~L.Inthispaper,wechosethevRnillinino8ceticacid-HC104s~nlngmethodastheoptimumconditiontodeterminethesap0niIlsofMomord/cac^讲L..aftercomparingwiththeHC104andH2S04systems.Everyfactorwasstudiedcarefully,theresul

4、tshowedthatthespectrophotometricconditionofdetemliIliIlgthesap0nsfromMomordicacharantia.L.was:0.2mLaceticacid,0.8mLHC104,5%vanillin-acetic8cid,temperature70℃.reactiontime20min.11regressivecurvewasobtainedusmsthe~poninfromtheMomord/cacharant/aL.,ithadgoodsi1ifican

5、ceandstability,anditalsohadgoodreproducibilityandofhighrneanrecoveryrateof100.78%,thecoefficientofvariationwasfoundtobe1.910%.Keywords:MomordicacharamiaL.;sapns;speetrophotometryMomordicacharantiaL.isafamiliarvegetable.Itbelongst0theCUCUrbitaceaefamily.I'm出tional

6、lythefruits.seedsandleavesareusedasananti—diabeticagent.InIndiawheretheplantisbetterknownas"Karela".itisusedasatonic.emeticandlaxativeagentL¨.瑚tionalChinesemedicineinchdesusesforthefruits,leavesandseedsingastroenteritis,diabetes,tLmlor$andsortieviralinfec—tionsL.

7、Forrecentyears.theoflicinalvalueswerecon—cemedmoreandn'lorebytheexpertsfromChinaandfor—eigncountries.TheresearchshowedthatthesaponinsinMomordicacharantiaL.havethehypoglucose,antitumor,antisenileactivityt3-5J.SaponinisachemicalcompositionwidelyexistinginChi—Blithe

8、traditionmedicine.Itisakindofglycoside.ItisReceivedSepte,,~cr7,2OO5;Accepted,cE2,2OO5*CorrespondingauthorTeh86-510-5869417;E-mail:stlMw@yahoo.odinnamedsaponins