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1、9-顺维甲酸对肺癌细胞生长特性的影响228中国分子心脏病学杂志2004年8月第4卷第4期(总第17期9.顺维甲酸对肺癌细胞生长特性的影响王虎于雪艳张士国胡国强任贵杰田克力[摘要]目的观察9.cis.RA对肺腺癌细胞株PG和A549生长抑制,诱导分化和凋亡的作用.方法将两株细胞分为1/zM,5p.M9.cis.RA组和对照组,应用细胞计数方法统计每组各时相细胞数,苏木精一伊红染色观察细胞分化情况,流式细胞仪分析检测细胞凋亡.结果(1)加入9.cis.RA12h后,PG细胞生长受抑程度显着高于对照组(P<0.O1),且5p.M组较1/zM组受抑程度
2、更高,而细胞变化不明显.(2)加入9.cis.RA24h后观察到PG细胞出现明显的分化特征,A549分化征象不明显,仅见分裂象减少.(3)加入9.cis.RA24h后PG细胞1/,M组和5M组凋亡率均显着高于对照组(P<0.01),且5M组凋亡率亦明显高于1M组(P<0.O1),A549细胞5M组凋亡率明显高于对照组(P<0.O1),而1M组与对照组无显着性差异.结论9.cis.RA对PG细胞株有明显的生长抑制,诱导分化和凋亡的作用,而对A.细胞的作用较弱,表明不同的细胞株对9.cis.RA的敏感性存在差异.[关键词]9.顺维甲酸;
3、细胞分化;细胞凋亡Effectsof9?ds-retinoicAcidonBiologicalCharacteristicsinLungCancerCellsWANGHUYUXue.yan2ZHANGShi.guoHUGuo.qiang4RENGui.jieTIANKe.1iSino-GermanLaboratoryforMolecularMedicine,FuWaiCardiovascularInstituteandHospital,CAMSandPUMC,Beijing100037,China[Abstract]ObjectiveToobserv
4、etheeffectsof9一cis—RAongrowthinhibition,differentiationandapopto-sisinductioninPGand4qhumanlungglandularcancercells.MethodsDividingPGand4qlungglandularcancercellseachintothreegroups:lM9一cis-RAgroup,5Mgroupandcontrolgroup.Cellnumbersofeachgroupindifferenttime—phasesWasbycellcoun
5、tmethods.ApoptosisWasdetectedbyflowcytometriccellcycleanal-ysis.Results(1)Form12hafterbeingtreatedwith9-cis-RA,thegrowthofPGcellWasinhibitedsignificantly,especiallyin5group,butA549cellhasnodistinctchange.(2)Afterbeingtreatedwith9-cis-RA,featuresofcelldifferentiationweredistinct
6、inPGcel1.A549cellhasnochange.(3)From24hafterbeingtreatedwith9-cis-RA,thepementagesofapoptoticcellswerehighersignificantlyinPGcell(P<0.01),comparedwithcontrolgroup.Andthepementagesofapoptosisin5taVlgroupWashighersignificantlythanthatin1/,Mgroup(P<0.01)+Thepementagesofapopt
7、osisin5taVlgroupWashigherdistinctthanthatincontrolgroupinA549cell,butthereWasnodifferencebetweenthe1groupandcontrolgroupinA549cel1.Conclusion9一cis—RAhaddistincteffectsongrowthinhibitiondiffer—emimionandapoptosisinductioninPGhumanlungglandularcancercellsandhadweakeffectsoncel1.[
8、Keywords]9一cis—retinoieacid;differentiation;apoptosis肺
