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1、T淋巴细胞在小鼠肾缺血再灌注损伤中的作用2O07年2月第15卷第1期中国实验动物ACTALABOBATOBIUMANIMALISSCIENTIASINICAFeb.,V01.152Oo7No.1,e)研究报告55蝗七,,蛉e,T淋巴细胞在小鼠肾缺血再灌注损伤中的作用陈恕求,陈明,张古田,李俊龙,彭涛,孙则禹(南京大学医学院附属鼓楼医院泌尿外科,南京210008)【摘要】目的研究T淋巴细胞在肾缺血再灌注损伤(ira)导致的急性肾损害中的作用.方法BALB/c小鼠和BALB/c裸小鼠各24只,分别随机分为A.一.组和
2、B.一.组,每组6只.双肾蒂阻断45min后恢复血流建立肾IRI模型,假手术对照组,lRI后24,48和72h时检测scr,尿蛋白定量及肾病理学,A组检测脾T细胞亚群;对比BALB/c小鼠和BALB/c裸小鼠的肾功能下降,组织学损害程度以及脾T淋巴细胞亚群变化.结果A2一.组和B2一.组均有Scr和尿蛋白定量明显升高(P<0.05),且A组损害程度明显重于B组(P<0.05);A2一.组出现典型的IRI组织损害表现(P<0.05),B2一.组无明显IRI组织损害(P>0.05);A2—3组
3、脾CD3'T细胞百分比较A.组升高(P<0.05),而CD4,CD8比值无明显变化(P>0.05).结论T淋巴细胞是小鼠肾IRI导致急性肾损害的重要病理生理学因素.【关键词】T淋巴细胞;再灌注损伤;肾;小鼠【中图分类号】R318.06【文献标识码】A【文章编号】1005.4847(2007)O1.0021.04PathophysiologicRoleofTLymphocytesinRenallschemia—ReperfusionInjuryinMiceCHENShu-qiu,CHENMing,ZHA
4、NGGu?tian,LIJun-long,PENGTao,SUNZe?yu(DepartmentofUrology,TheAffiliatedDrumTowerHospitalofNanjingUniversityMedicalSchool,Nanjing210008,China)【Abstract】ObjectiveToinvestigatethepathophysiologicroleofTlymphocytesinrenalischemia-reperfusioninjuryinmice.MethodsBA
5、LB/cwild?mice(groupA,n=24)andBALB/cnude?mice(groupB,n=24)weredividedintofourgroups(n=6),respectively,thegroupsAltoA4andgroupsBltoB4.Bilateralrenalpedicleswereoccludedbymicrovascularclampsfor45minutes.Thenthekidneyswerereperfused.Sampleswerecollectedatshamoper
6、ationand24h,48hand72hafterIBI.Serumcreatinine(Scr),urineproteinconcentration,renalhistologyandsplenicCD3/CD4/CD8positivecells(groupA)wereexamined,respectively.ResultsScrandurineproteinconcentrationincreasedandhistologicalinjuryWasobservedingroupA2—4andB2—4.Th
7、einjuryingroupAweremoreseverethanthatingroupB(P<0.05).FACSanalysisshowed,thatincomparisonwithgroupAl,percentageofsplenicCD3TcellsingroupA2andA3increasedsignificantly(P<0.05),butasimilarratioofCIM/CD8'betweengroupAIandgroupA2—3(P夕0.05)).ConclusionTlympho
8、cytesplayanimportantpathophysiologicroleinrenalischemia?reperfusioninjury,andmaymediatepost?ischemicdeclineinrenalfunctionandleadto~stologicalinjury.【Keywords】Tlymphocytes;Reperfusioninju
