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1、StudyonGeneticDiversityamongSoybeaninHeilongjiangProvinceJune20o6JournalofNortheastAgnculturalUniversityVo1.13.No.1ll—l5ArticleID:1006—8104(2006)一01—0011—05StudyonGeneticDiversityamongSoybeaninHeilongjiangProvinceHUGuofu',HUXiaomei,CHANGYing',LIUDi.,HUBaozhong.(1.CoHegeofLifeScience
2、,NortheastAgriculturalUniversity,HarbinHeilongjiang150030,PRC;2.SchoolofLifeandHealthSciences,AstonUniversity,BirminghamB47ET,UK)Abstract:Geneticcharactersof10soybeanbreedsinHeiloniangProvincewerestudiedusingRAPDtechnique.Wecalculatedthegeneticsimilarityandgeneticdistance,madetheclu
3、steranalysis,andalsostudiedthegeneticrelationshipamongthe10breeds.Wefound93amplificationsegmentsbyRAPDanalysis.inwhichtherewere70polymorphismsegments,75.26%ofamplificationsegments,using13randomprimers,11,12,5,4,7,8,3,7,2,5,10,10,9ampl讯catedbandswerefound.Therewere7.15bandsperprimeri
4、naverage.Thegeneticsimilarityamongthebreedsrangedm0.6848to0.9019.andthegeneticdistancerangedm0.0981to0.3152.Geneticrelationshipbetween90035and9674wastheclosest,andbetweenHeireng27and90035wasthefa~hest.Proteinanalysisrevealedthattheclosestgeneticrelationshipwasbetween90035and90024.an
5、dthefa#chestwasbetween1330andSui4.andbetween1330andHeinong39.Keywords:soybean;RAPD;protein;geneticcharacterCLCnumber:$565.1:Q78Documentcode:AHeilongjiangProvince,whichpossessesabundantsoybeangermplasm,isthemainsoybeanproducingre—gioninChina【lJ.Thecomplexityofthesoybeankaryo-typebrin
6、gsmanydifficultiestostudytheclassificationofsoybeanbytraditionalmethod.Andduetohighselfcrossfrequencyandnarrowgeneticbackground,itisdifficulttoanalyzethepolymorphismofsoybeanbyisoenzymemethodandRFLP【.AsKishaandDierspointedout,theRFLPdis—tancebetweenparentstrainhassignificantcorrelat
7、ionwithgeneticvariationofsubsequentgeneration,al—thoughtherelatedcoe佑cientissmallt~.Itisusefultostudythegeneticcharacterofsoybeangermplasmforprotecting,developingandimprovingthesoybeanbreedsn.Inthisstudy,wetriedRAPDandproteinelectrophoresistoanalyzegeneticvariationsofsomebreedsfromH
8、eilongjiangProvince
