evaluation of genetic diversity among jordanian fig germplasm accessions by

evaluation of genetic diversity among jordanian fig germplasm accessions by

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1、ScientiaHorticulturae147(2012)8–19ContentslistsavailableatSciVerseScienceDirectScientiaHorticulturaejournalhomepage:www.elsevier.com/locate/scihortiEvaluationofgeneticdiversityamongJordanianfiggermplasmaccessionsbymorphologicaltraitsandISSRmarkersDua’aa,bb,∗a,cAlmajali,A

2、delH.Abdel-Ghani,HusseinMigdadiaNationalCenterforAgriculturalResearchandExtension,Al-Baq‘a,Amman,JordanbMutahUniversity,FacultyofAgriculture,DepartmentofPlantProduction,P.O.Box7,Al-Karak,JordancCollegeofFoodandAgriculturalSciences,KingSaudUniversity,Riyadh,SaudiArabiaar

3、ticleinfoabstractArticlehistory:ThisstudydescribesphenotypicvariationinleafandfruittraitsandinvestigatesgeneticrelationshipsReceived16July2012among24landrace(FicuscaricaL.sativa)and6wildform(F.caricaL.caprificus)accessionsoffigusinginterReceivedinrevisedform23August2012si

4、mplesequencesrepeat(ISSR)markers.Forphenotypictraits,pair-wiseEuclideandistancesrangedfromAccepted24August20120.90to9.80.Principalcomponentanalysisrevealedthatthefirstfivecomponentsexplained74.14%ofthetotalKeywords:morphologicalvariation,wherefruittraitscontributedmostoft

5、hetotalvariation.Nei’sgeneticdis-tancebasedonISSRdatarangedfrom0.00to0.83,suggestingthatthecollectedaccessionsaregeneticallyGeneticdiversitydiverse.diversityUPGMAclusteringbasedonphenotypictraitscomparedwiththatbasedonISSRdatawerenotPhenotypicconsistent,however,somecomm

6、ongroupingswereobservedinISSRandphenotypictraits.Inmostcases,ISSRaccessionsFigcollectedfromthesamelandraceorfromfigwildformstendedtoclustertogether,confirmingthatnamesgivenbyfarmerstothecollectedlandraceaccessionsareconsistentandconfirmingalsothecommongeneticbackgroundofwi

7、ldfigaccessions.HighphenotypicandISSRvariabilityindicatethatfigcollectionsusedinthisstudyincluderichandvaluablegenesforfigbreeding.ISSRscreeningrevealedthepresenceofthesameISSRallelicprofileforaccessionsfromthesamelandraceorwildformaccessions,indicatingthatISSRcanprovidethe

8、basicinformationnecessarytohelpgenebankstoconservematerialsfromdifferentgeneticbackgroundratherthanduplicatesf

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