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1、BiochemicalSystematicsandEcology43(2012)73–79ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoAssessmentofgeneticdiversityinAchilleamillefoliumaccessionsfromIranusingISSRmarkeraa,*abaMostafaFarajpour,Mo
2、hsenEbrahimi,RezaAmiri,RasoulGolzari,SepidehSanjariaDepartmentofAgronomyandPlantBreeding,CollegeofAbouraihan,UniversityofTehran,Tehran,IranbDepartmentofAgronomyandPlantBreeding,CollegeofAgriculture,UniversityofZanjan,Zanjan,IranarticleinfoabstractArticlehistory:Thirty-sevenaccession
3、sofAchilleamillefoliumcollectedfromprovincesinIran,wereReceived20April2011analyzedusinginter-simple-sequence-repeat(ISSR)markerstoevaluatetheirvariability.Accepted19February2012AtotalofsevenISSRprimersgenerated72amplifiedfragments,mostofwhichwereAvailableonline28March2012polymorphic.
4、TheJaccardsimilarityindices,asbasedontheISSRprofiles,weresubjectedtoacompletelinkageanalysis,andthedendrogramrevealedsixgroups.TheresultsoftheKeywords:clusteringshowedthatA.millefoliumsubspecieselbursensis(endemictoNorthernIran)isGeneticdiversityseparatedfromothergenotypesinthedendro
5、gram.ThePrincipleComponentAnalysisAchilleamillefolium(PCA)confirmedtheresultsoftheclustering.TostudythemorphologicalandphonologicalISSRmarkerPCAtraitsoftheseplants,wecultivatedtheminarandomizedcompleteblockdesignwiththreereplications,andthesetraitsweremeasuredduringtheearlystageofflow
6、ering.Theanalysisofvarianceshowedthatallofthemeasuredtraitshavevariationamongtheseplants.TheobjectiveofthepresentstudywastheidentificationofthegeneticdiversityamongA.millefoliumgenotypesusingISSRmarkersandselectedmorphologicalandphonologicaltraits.Ó2012PublishedbyElsevierLtd.1.Introd
7、uctionYarrowbelongstooneoftheyoungestevolutionarygeneraoftheAsteraceaefamily(Farajpouretal.,2011).Theseplantsareperennialherbsandwell-knownmedicinalplants,whicharewidelyusedinfolkmedicineagainstgastrointestinaldisordersthatcausealackofappetite(Bimbiraiteetal.,2008_).InIran,thenatura
8、lhabitatsofthesepla