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1、StudyonmitochondrialDNAdiversityamong7inbredstrainsofmice164JournalofMedicalCollegesofPLA2006;21(3)StudyonmitochondrialDNAdiversityamong7inbredstrainsofmiceDAIJi—gang(戴纪刚),XIAOYing—bin(肖颖彬),ZHANGGuo—qiang卜.(张国强),MINJia—xin(闵家新),WEIHong(魏泓)DepartmentofThoracicSurgery,XinqiaoHospital,丁^MilitaryMedica
2、lUniversity,Chongqing400037,ChinaCenterofLaboratoryAnimal,ThirdMilitaryMedicalUniversity,Chongqing400038,China[Abstract]Objective:TostudythegeneticvariationofmitochondrialDNA(mtDNA)amongcommonlaboratorystrainsofinbredmice.Methods:ThegeneticpolymorphismofmtDNAamong4classicallabora—torystrainsofinbre
3、dmiceand3inbredstrainsofmiceestablishedinChinawasanalyzedbypolymerasechainreactioncoupledwithrestrictionfragmentlengthpolymorphism(PCR—RFLP)andPCRcoupledwithsingle—strandedconformationpolymorphism(PCR—SSCP).Results:WithregardtotheD—loop(Displace—mentloop,D—loop),tRNA'.,andND3(NADHdehydrogenasesubun
4、it3.ND3)genefragmentsofmtDNAfromthesemice,novariationwasrevealedbyPCR—RFLPat46restrictionenzymesites.FurtheranalyzedbyPCR—SSCP,theD—loop5fragmentand3endfragmentofmtDNAfromthesemicealsoshowednogeneticvariation.Conclusion:OwingtomaternalmodeofinheritanceofmtDNA.theresultsindicatethatthesecommoninbred
5、strainsofmicesharethesamematernallineage.[Keywords]inbredstrains;mtDNA;polymerasechainreactioncoupledwithrestrictionfragmentlengthpolymorphism;PCRcoupledwithsingle—strandedconformationpolymorphism;polymorphismThehighstabilityandpure—lineageintheheredityofinbredstrainsofmicemakesitoneofthecommonlabo
6、ratoryanimalswhicharewidelyusedinbiomedicalresearch.Thefactthatcompleteinbreedingistoleratedhasallowedtheestablish—mentofhundredsofinbredstrainsE.Mouseinbredstrainsmaybedividedintotwogroups,classicalandwild—derived.Wild—derivedgroupoflaboratorymiceconsistsofseveraldozeninbredstrainsthathavebeenderi
7、vedfromwildmicetrapped,atdif—ferenttimesandlocations,fromdifferentpopula—tionsE.TheseincludeinbredstrainsestablishedfrommicebelongingtoseveralMusspeciesandin—bredstrainsderivedfromdifferentMusmusculussubspe
