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1、BiotechnologyAdvances26(2008)424–435ContentslistsavailableatScienceDirectBiotechnologyAdvancesjournalhomepage:www.elsevier.com/locate/biotechadvResearchreviewpaperRoleofbiotechnologicalinterventionsintheimprovementofcastor(RicinuscommunisL.)andJatrophacur
2、casL.a,⁎,T.P.ReddybcM.Sujatha,M.J.MahasiaDirectorateofOilseedsResearch,Rajendranagar,Hyderabad500030,IndiabDepartmentofGenetics,OsmaniaUniversity,Hyderabad500007,IndiacKenyaAgriculturalResearchInstitute(KARI),57811-00200Nairobi,KenyaARTICLEINFOABSTRACTArt
3、iclehistory:CastorandJatrophabelongtotheEuphorbiaceaefamily.ThisreviewhighlightstheroleofbiotechnologicalReceived6December2007toolsinthegeneticimprovementofcastorandjatropha.CastorismonotypicandbreedingprogrammeshaveReceivedinrevisedform1April2008mostlyre
4、liedonthevariabilityavailableintheprimarygenepool.ThemajorconstraintslimitingprofitableAccepted9May2008cultivationare:vulnerabilitytoinsectpestsanddiseases,andthepresscakeistoxicwhichrestrictitsuseasAvailableonline22May2008cattlefeed.Conventionalbreedingte
5、chniqueshavelimitedscopeinimprovementofresistancetobioticstressesandinqualityimprovementowingtolowgeneticvariabilityforthesetraits.GeneticdiversitywasKeywords:Geneticdiversityassessedusingproteinbasedmarkerswhileuseofmolecularmarkersisatinfancy.Invitrostu
6、diesincastorGenetictransformationhavebeensuccessfulinshootproliferationfrommeristematicexplants,butnotcallus-mediatedGenomicsregeneration.GenetictransformationexperimentshavebeeninitiatedfordevelopmentofinsectresistantMolecularmarkersandricin-freetransgen
7、icswithverylowtransformationfrequency.IntropicalandsubtropicalcountriesTissueculturejatrophaisviewedasapotentialbiofuelcrop.Thelimitationsinavailablegermplasminclude;lackofWidehybridizationknowledgeofthegeneticbase,pooryields,lowgeneticdiversityandvulnera
8、bilitytoawidearrayofinsectsanddiseases.Greatscopeexistsforgeneticimprovementthroughconventionalmethods,inducedmutations,interspecifichybridizationandgenetictransformation.Reliableandhighlyefficienttissuecultureprotoco
