红腺忍冬叶抗氧化有效部位中总多酚的含量测定.pdf

《红腺忍冬叶抗氧化有效部位中总多酚的含量测定.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、L0c卫z广oN工mJl》z0n工一zm∽m王m0一n广cz一

2、0％G醇沉淀物和滤液中总多酚的含量。[结果】测得总多酚含量在6g·mL-~16I．Lg·mE-范围内具有良好的线性关系(r=0．9996)，重复I~RSD为O．31％(n=6)，平均加样回收率为98．67％，RSD为2．66％(n=9)。不同批次红腺忍冬叶间测得总多酚含量差别较大；同批次红腺忍冬叶中，总多酚含量为水提液>水提液30％乙醇沉淀后滤液>水提液30％乙醇沉淀。[结论】该方法简单、准确、重复性好，可用于测定红腺忍冬叶抗氧化有效部位中总多酚的含量。红腺忍冬叶抗氧化有效部位中总多酚含量为水提液>水提液30％乙醇沉淀后滤液>水提液

3、30％乙醇沉淀。关键词：红腺忍冬叶；抗氧化有效部位；总多酚；含量测定；Fofin—Ciocaileu法中图分类号：R282．71文献标识码：A文章编号：1005-5509(2014)03-o316-06ContentDeterminationofTotalPolyphenolinAntioxidantEfectiveFractionofLeavesofLoniceraHypoglaucaMiq．GeShanshan，ZhuYing，GuoXinyan，etalCollegeofPharmacyofZhejiangChineseMed

4、icalUniversity,Hangzhou(310053)Abstract：[Objective]ToestabhshaFolin—CiocaileumethodfordeterminationofthecontentsoftotalhydroxybenzeneinantioxidantefectivefractionofleavesofLoniceraHypoglaucaMiq．whichisusedintheexplorationofthecontentchangesoftotalhydroxybenzeneinantiox

5、idantefectivefractionofleavesofLoniceraHypoglaucaMiq．ofdiferentbatches．[Methods]Themethodologyofthemethodfordeterminationofthecontentsoftotalhydroxybenzeneinwaterex~actionofleavesofLoniceraHypoglaucaMiq．wasinvestigatedbyFohn—Ciocafleumethod．Thecontentsoftotalhydroxyben

6、zeneinwaterextractionsortheprecipitationandfiltrateof30％ethanolprecipimtingactionfrom12batchesofleavesofLoniceraHypoglaucaMiq．weredetermined．[ResultsJThemethodolO~calsmdyshowedthatthelinearrangeoftotalhydroxybenzenewa$6g,g。mL一16g‘n1Lr=0．9996)．TherepetitivenesstkSDoftot

7、alpolyphenolwasO．31％(n6)．Inaddition，theaveragerecoveryoftotalpolyphenolwas9867％，withRSD2．66％(n9)．ThetotalhydroxybenzenecontentsindiferentbatchesextractionofleavesofLoniceraHypoglaucaMiq．werequitediferent．WhilethecontentsoftotalhydroxybenzeneinthesameleavesofLoniceraHyp

8、oglaucaMiq．were：waterextraction>thefiltrateof30％ethanolprecipitatingaction>theprecipitationof30％ethanolprecipitatinga