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《耐碳青霉烯肺克雷伯菌和大肠埃希菌耐药机制研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、国际检验医学杂志2014年1月第35卷第2期IntJLabMed,January2014,Vo1.35,No.2·l65··临床检验研究论著·耐碳青霉烯肺克雷伯菌和大肠埃希菌耐药机制研究梁权辉,徐韫健(1.广州医科大学第一临床学院检验系O9级,广东广州510120;2.广州医科大学附属第一医院检验科,广东广州510120)摘要:目的分析该院-I盏床分离的可疑产碳青霉烯酶肺炎克雷伯菌和大肠埃希菌的耐药性与耐药机制。方法采用VITEK一2全自动细菌鉴定仪检测其对21种抗菌药物的药敏结果,采用改良Hodge试验进行碳青霉烯酶筛选,用聚合酶链反应检测碳青霉烯
2、酶KPC基因、外膜孔道蛋白(OmpK35和OmpK36)编码基因、转座子tnpA、tnpU基因。结果47株可疑产碳霉烯酶肺炎克雷伯茵和大肠埃希茵对青霉素类、头孢菌素类、喹诺酮类抗菌药物的耐药率高达8O以上,对亚胺培南、美罗培南、丁胺卡那霉素耐药率为35以下;耐亚胺培南菌株对头孢哌酮/舒巴坦、头孢美唑、头孢替坦、头孢吡肟、哌拉西林/他唑巴坦、庆大霉素、妥布霉素、美罗培南、厄他培南的耐药率明显高于敏感菌株,二者差异具有统计学意义(P3、K35、OmpK36基因缺失率分别为38.30、65.96;转座子tnpA、tnpU检出率分别为4.25和38.3O。结论产碳青霉烯酶并非茵株耐碳青霉烯类药物的主要原因.尚存在其他耐药机制。关键词:碳青霉烯酶;克雷伯菌,肺炎;大肠杆菌;抗药性,微生物DOI:lO.3969/i.issn.1673—4130.2O14.02.016文献标识码:A文章编号:1673—4130(20l4)020l6504StudyonresistantmechanismofcarbapenemresistantKlebsiellapneumoniaeandEscherich4、iacoliLiangQuanhui,XuYunjian(1.DepartmeritoyMedicalLaboratory,theFirstClinicCollege,GuangzhouMedicalUniversity,Guangzhou,Guangdong510120,China;2.Department0/MedicalLaboratory,theFirstAJl,、iliatedHospital,GuangzhouMedicalUniversity,Guangzhou,Guangdong510120,China)Abstract:Object5、iveToinvestigatethedrugresistanceandtheresistantmechanismofsuspiciouscarbapenemasesinKiebsiellapneumoniaeandEscherichiacoliclinicalisolates.MethodsAllbacteriawereisolatedfromtheFirstAffiliatedHospilalofGuang—zhouMedicalCollege,andantimicrobialsusceptibilitywasdonebyVITEK~2autom6、aticbacteriumidentifyinganddrugsel~sitivityanalyzingsystems.ThescreeningofthecarbapenemasewasdetectedbythemodifiedHodgetest,andthepolymerasechainreaction(PCR)wascarriedouttoamplifycarbapenemhydrolyzinggene(KPC),outermembraneproteingenes(OmpK35and()mpK36).transposontnpAandtnpU.R7、esultsAmong47strainsofsuspiciouscarbapenemasesinKlebsiellapneumoniaeandEschop‘ichiacoli,thehigherdrugresistanceratestothemwerepenicillins,cephalosporinsandquinolones,whichweremorethan80.1hcresistanceratesforimipenem,meropenemandamikacinwerelessthan35.Inadditiontotheimipenem—res8、istantstrains,theresistanceratestocefoperazone/sulbact
3、K35、OmpK36基因缺失率分别为38.30、65.96;转座子tnpA、tnpU检出率分别为4.25和38.3O。结论产碳青霉烯酶并非茵株耐碳青霉烯类药物的主要原因.尚存在其他耐药机制。关键词:碳青霉烯酶;克雷伯菌,肺炎;大肠杆菌;抗药性,微生物DOI:lO.3969/i.issn.1673—4130.2O14.02.016文献标识码:A文章编号:1673—4130(20l4)020l6504StudyonresistantmechanismofcarbapenemresistantKlebsiellapneumoniaeandEscherich
4、iacoliLiangQuanhui,XuYunjian(1.DepartmeritoyMedicalLaboratory,theFirstClinicCollege,GuangzhouMedicalUniversity,Guangzhou,Guangdong510120,China;2.Department0/MedicalLaboratory,theFirstAJl,、iliatedHospital,GuangzhouMedicalUniversity,Guangzhou,Guangdong510120,China)Abstract:Object
5、iveToinvestigatethedrugresistanceandtheresistantmechanismofsuspiciouscarbapenemasesinKiebsiellapneumoniaeandEscherichiacoliclinicalisolates.MethodsAllbacteriawereisolatedfromtheFirstAffiliatedHospilalofGuang—zhouMedicalCollege,andantimicrobialsusceptibilitywasdonebyVITEK~2autom
6、aticbacteriumidentifyinganddrugsel~sitivityanalyzingsystems.ThescreeningofthecarbapenemasewasdetectedbythemodifiedHodgetest,andthepolymerasechainreaction(PCR)wascarriedouttoamplifycarbapenemhydrolyzinggene(KPC),outermembraneproteingenes(OmpK35and()mpK36).transposontnpAandtnpU.R
7、esultsAmong47strainsofsuspiciouscarbapenemasesinKlebsiellapneumoniaeandEschop‘ichiacoli,thehigherdrugresistanceratestothemwerepenicillins,cephalosporinsandquinolones,whichweremorethan80.1hcresistanceratesforimipenem,meropenemandamikacinwerelessthan35.Inadditiontotheimipenem—res
8、istantstrains,theresistanceratestocefoperazone/sulbact
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