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1、E5半定量RT-PCR法测定TRAIL,survivin在同癌组织中表达及其意义[摘要]目的检测肿瘤坏死因子相关凋亡诱导配体(tumornecrosisfactor-relatedapoptosis-inducingligand,TRAIL)及抑制凋亡基因家族成员survivin在胃癌组织中的表达,并探讨其意义。方法利用半定量逆转录聚合酶链反应(RT-PCR)法检测82例胃腺癌及对应的正常胃组织中TRAIL和survivin的表达。结果TRAIL在正常胃组织中的表达阳性率及相对表达量均明显高于癌组织(P〈0.05);而survivin的表达则与TRA
2、IL表达相反,正常胃组织中不表达,在胃癌组织中的表达阳性率为62.2%oTRAIL和survivin表达量与肿瘤的分化程度有关,分化程度越低,TRAILmRNA表达量越低,而survivinmRNA表达量越高,高分化胃腺癌与低分化胃腺癌间差异有统计学意义(P<0.05)。结论TRAIL和survivin在胃癌凋亡过程中起着重要的作用,TRAIL可诱导凋亡,survivin则抑制凋亡发生。。[关键词]胃癌;肿瘤坏死因子相关凋亡诱导配体;survivin;逆转录聚合酶链反应。[中图分类号]R735.2;Q786[文献标识码]A[文章编号]1671-756
3、2(2007)02-0125-04DetectionofTRAILandsurviviningastric❷carcinomabysemiquantitiveRT-PCR。。WANGXiao-hongl,WANGSuo-rongl,ZHOUQing2,ZHUChun-meil,CAOSuo-yu^l(1.DepartmentofGastroenterology,thePeople^sHospitalofDanyang,Danyang212300,China;^2.DepartmentofPathology,theAffiliatedHospitalo
4、fNantongUniversity,Nantong226001,China)。^Abstract:ObjectiveToinvestigatetheexpressionoftumornecrosisfactor-relatedapoptosis-inducingligand(TRAIL)andsurviviningastriccarcinoma.MethodsExpressionofTRAILandsurvivinwereassayedbysemiquantitivereversetranscriptasepolymerasechainreacti
5、on(RT-PCR)in82casesofgastriccarcinomaandnormalgastrictissues.ResultsThepositiverateandexpressionquantityofTRAILinnormaltissueswerehigherthanthoseincancertissues(P<0.05).However,thesurvivinwasonlyexpressedincancertissues,thepositiveratewas62.2%.TheexpressionquantityofTRAILandsur
6、vivinwascorrelatedwithhistologicalgrade,theexpressionofTRAILwashigherinwell-differentiatedgastricadenocarcinomathanthatinpoorlydifferentiatedgastricadenocarcinomawhiletheexpressionofsurvivinwaslower(P<0.05).ConclusionTRAILcaninduceapoptosisandsurvivincandepressthehappeningofapo
7、ptosis,whichmaybeoneofthemechanismsbywhichgastriccarcinomadevelops.。Keywords:gastriccarcinoma;tumornecrosisfactor-relatedapoptosis-inducingligand;survivin;reversetranscriptasepolymerasechainreaction。(ModernMedicalJournal,2007,35:125-128)肿瘤坏死因子相关的凋亡诱导配体(tumornecrosisfacter-relat
8、edapoptosis-inducingligand,TRAIL)是TNF超家族中的成员,属II型跨膜蛋白,