半定量rt-pcr法测定trail、survivin在胃癌组织中表达及其意义

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1、半定量RT-PCR法测定TRAIL、survivin在胃癌组织中表达及其意义[摘要]目的检测肿瘤坏死因子相关凋亡诱导配体（tumornecrosisfactor-relatedapoptosis-inducingligand,TRAIL）及抑制凋亡基因家族成员survivin在胃癌组织中的表达,并探讨其意义。方法利用半定量逆转录聚合酶链反应（RT-PCR）法检测82例胃腺癌及对应的正常胃组织中TRAIL和survivin的表达。结果TRAIL在正常胃组织中的表达阳性率及相对表达量均明显高于癌组织(P<0.05)；而survivin的表达则与TRAIL表达相反，正常胃组织中不表

2、达，在胃癌组织中的表达阳性率为62.2%。TRAIL和survivin表达量与肿瘤的分化程度有关，分化程度越低，TRAILmRNA表达量越低，而survivinmRNA表达量越高，高分化胃腺癌与低分化胃腺癌间差异有统计学意义(P<0.05)。结论TRAIL和survivin在胃癌凋亡过程中起着重要的作用，TRAIL可诱导凋亡，survivin则抑制凋亡发生。�[关键词]胃癌;肿瘤坏死因子相关凋亡诱导配体；survivin；逆转录聚合酶链反应�[中图分类号]R735.2;Q786[文献标识码]A[文章编号]1671-7562（2007）02-０１２５-０４�� 12Detect

3、ionofTRAILandsurviviningastric�carcinomabysemiquantitiveRT-PCR��WANGXiao-hong1,WANGSuo-rong1,ZHOUQing2，ZHUChun-mei1,CAOSuo-yu�1(1.DepartmentofGastroenterology,thePeople�sHospitalofDanyang，Danyang212300，China;�2.DepartmentofPathology,theAffiliatedHospitalofNantongUniversity，Nantong226001，Chi

4、na)��Abstract:ObjectiveToinvestigatetheexpressionoftumornecrosisfactor-relatedapoptosis-inducingligand(TRAIL)andsurviviningastriccarcinoma.MethodsExpressionofTRAILandsurvivinwereassayedbysemiquantitivereversetranscriptasepolymerasechainreaction(RT-PCR)in82casesofgastriccarcinomaandnormalgas

5、trictissues.ResultsThepositiverateandexpressionquantityofTRAILinnormaltissueswerehigherthanthoseincancertissues(P<0.05).However，thesurvivinwasonlyexpressedincancertissues,thepositiveratewas62.2%.TheexpressionquantityofTRAILandsurvivinwascorrelatedwithhistological12grade，theexpressionofTRAIL

6、washigherinwell-differentiatedgastricadenocarcinomathanthatinpoorlydifferentiatedgastricadenocarcinomawhiletheexpressionofsurvivinwaslower(P＜0.05).ConclusionTRAILcaninduceapoptosisandsurvivincandepressthehappeningofapoptosis，whichmaybeoneofthemechanismsbywhichgastriccarcinomadevelops.�Keywo

7、rds:gastriccarcinoma;tumornecrosisfactor-relatedapoptosis-inducingligand;survivin;reversetranscriptasepolymerasechainreaction�(ModernMedicalJournal,2007,35:125-128)��肿瘤坏死因子相关的凋亡诱导配体(tumornecrosisfacter-relatedapoptosis-inducingligand,TRAIL）是TNF超家族中的成员，属Ⅱ