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ID:11337866
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页数:12页
时间:2018-07-11
《半定量rt-pcr法测定trail、survivin在胃癌组织中表达及其意义》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、半定量RT-PCR法测定TRAIL、survivin在胃癌组织中表达及其意义[摘要]目的检测肿瘤坏死因子相关凋亡诱导配体(tumornecrosisfactor-relatedapoptosis-inducingligand,TRAIL)及抑制凋亡基因家族成员survivin在胃癌组织中的表达,并探讨其意义。方法利用半定量逆转录聚合酶链反应(RT-PCR)法检测82例胃腺癌及对应的正常胃组织中TRAIL和survivin的表达。结果TRAIL在正常胃组织中的表达阳性率及相对表达量均明显高于癌组织(P<0.05);而survivin的表达则与TRAIL表达相反,正常胃组织中不表
2、达,在胃癌组织中的表达阳性率为62.2%。TRAIL和survivin表达量与肿瘤的分化程度有关,分化程度越低,TRAILmRNA表达量越低,而survivinmRNA表达量越高,高分化胃腺癌与低分化胃腺癌间差异有统计学意义(P<0.05)。结论TRAIL和survivin在胃癌凋亡过程中起着重要的作用,TRAIL可诱导凋亡,survivin则抑制凋亡发生。�[关键词]胃癌;肿瘤坏死因子相关凋亡诱导配体;survivin;逆转录聚合酶链反应�[中图分类号]R735.2;Q786[文献标识码]A[文章编号]1671-7562(2007)02-0125-04�� 12Detect
3、ionofTRAILandsurviviningastric�carcinomabysemiquantitiveRT-PCR��WANGXiao-hong1,WANGSuo-rong1,ZHOUQing2,ZHUChun-mei1,CAOSuo-yu�1(1.DepartmentofGastroenterology,thePeople�sHospitalofDanyang,Danyang212300,China;�2.DepartmentofPathology,theAffiliatedHospitalofNantongUniversity,Nantong226001,Chi
4、na)��Abstract:ObjectiveToinvestigatetheexpressionoftumornecrosisfactor-relatedapoptosis-inducingligand(TRAIL)andsurviviningastriccarcinoma.MethodsExpressionofTRAILandsurvivinwereassayedbysemiquantitivereversetranscriptasepolymerasechainreaction(RT-PCR)in82casesofgastriccarcinomaandnormalgas
5、trictissues.ResultsThepositiverateandexpressionquantityofTRAILinnormaltissueswerehigherthanthoseincancertissues(P<0.05).However,thesurvivinwasonlyexpressedincancertissues,thepositiveratewas62.2%.TheexpressionquantityofTRAILandsurvivinwascorrelatedwithhistological12grade,theexpressionofTRAIL
6、washigherinwell-differentiatedgastricadenocarcinomathanthatinpoorlydifferentiatedgastricadenocarcinomawhiletheexpressionofsurvivinwaslower(P<0.05).ConclusionTRAILcaninduceapoptosisandsurvivincandepressthehappeningofapoptosis,whichmaybeoneofthemechanismsbywhichgastriccarcinomadevelops.�Keywo
7、rds:gastriccarcinoma;tumornecrosisfactor-relatedapoptosis-inducingligand;survivin;reversetranscriptasepolymerasechainreaction�(ModernMedicalJournal,2007,35:125-128)��肿瘤坏死因子相关的凋亡诱导配体(tumornecrosisfacter-relatedapoptosis-inducingligand,TRAIL)是TNF超家族中的成员,属Ⅱ
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