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1、一株中度嗜盐蜡样芽泡杆菌的分离鉴定及其对苯胺的降解特性摘要：从江苏省连云港市某化工染料厂排污管道的污水中驯化、分离得到1株能以苯胺为惟一碳源的降解菌株HYHB-3,经形态学特征及生理生化特性观察，结合16SrRNA基因序列分析，初步鉴定为蜡样芽泡杆菌(Bacilluscereus),并研究其对苯胺的降解特性和影响因素。结果表明，菌株HYHB-3为该菌株属中度嗜盐，最佳生长温度25°C,超过35°C停止生长；苯胺浓度100mg/L是HYHB-3菌株降解最适浓度，降解率达77.9%,250mg/L为饱和浓度；随时间延长降解效率有增加趋势；菌株HYHB-3降解苯胺的最适温度是25°C,降解率达69.
2、9%,.R对低温条件具有一定的适应性，15〜20°C条件下均有一定的降解；降解苯胺最适pH为8,降解率达到最大值，达58.4%；在NaCl含量1%〜9%的范围内均有一定的降解，3%、5%时降解率均超过50%以上，9%时仍然有11.7%的降解率。苯胺浓度250mg/L以上则可以抑制该菌的生长。关键词：蜡样芽泡杆菌(Bacilluscereus)；分离鉴定；苯胺；降解中图分类号:S852.61+6文献标识码:A文章编号:0439-8114(2014)02-0308-04IsolationandIdentificationofaModeratelyHalophilicBacilluscereusan
3、dTtsDegradationCharacteristiconAnilineWANGIlong-bini,2,SONGXiu-meil,ZHENGJin-long1,YANBin-lun2(1.MarineCollege,HuaiHaiInstituteofTechnology,Lianyungang222005,Jiangsu,China；2.JiangsuKeyLaboratoryofMarineBiotechnology,Lianyungang222005,Jiangsu,China)Abstract：AhighlyefficientdegradationbacteriumIIYIIB-
4、3withanilineasitssolecarbonsourcewasisolatedfromthesewagepipeofachemicaldyefactoryinLianyungang,JiangsuProvince.Accordingtothemorphological,physiologicalandbiochemicalcharacteristics,combinedwith16SrRNAsequenceanalysis,itwasidentifiedasBacilluscereus.Itsdegradationcharacteristicsonanilineandfactorsi
5、nfluencingthedegradationwerestudied.TheresultsshowedthatthestrainHYHB-3wasmoderatelyhalophilicwithoptimumgrowthtemperatureof25°C.Itmightstopgrowingover35°C.Theanilinemassconcentrationof100mg/LwastheoptimaldegradationconcentrationofstrainI1YI1B-3,withthehighestdegradationrate77.9%.250mg/Lwasthesatura
6、tionconcentration.Theoptimumdegradationtimewas96〜120h.Thedegradationefficiencyincreasedwithtime.TheoptimumtemperatureofanilinedegradationofStrainHYHB-3was25°Cwithdegradationrate69.9%.Ithadcertainadaptabilitytothelowtemperatureconditionwith15〜20°C.TheoptimumpHofanilinedegradationwas8withmaximumanilin
7、edegradationrate58.4%.TherewasacertaindegradationwhentheNaClcontentof1%〜9%.Thedegradationratewereover50%whenNaClwas3%and5%.Thedegradationratewas11.7%whenNaClwas9%.Theanilinemassconcentrationof250mg/Lc
