α-硫辛酸对2型糖尿病大鼠肝脏脂质沉积的改善作用-论文.pdf

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1、中华临床医师杂志f电子版)2013年12月第7卷第23期ChinJClinicians(ElectronicEdition)．December1．2013．v01．7．No．23·187·基础论著●．硫辛酸对2型糖尿病大鼠肝脏脂质沉积的改善作用．史治宙郭中秋谭娅琴万新红武革陈晓兵黄继平陈玉华【摘要】目的探讨硫辛酸(a．1ipoicacid，ALA)对高脂联合链脲佐菌素(STZ)诱导的SD大鼠肝脏脂质沉积的影响。方法健康雄性8周龄SD大鼠45只，随机分为正常对照组15只，造模组3O只，造模组应用高糖高脂饮食加30mg／kgSTZ诱

2、发2型糖尿病模型，成模大鼠再随机分成非药物干预糖尿病组(DM组，”=15)和．硫辛酸干预组(ALA组，60mg／kg，=15)，干预8周。干预结束后留取血清和肝脏组织，行生化及氧化应激指标检测，组织形态学检测，并应用Westernblot的方法测定大鼠肝脏组织脂代谢相关蛋白固醇调节元件结合蛋白．1(sterolregulatoryelementbindingprotein．1，SREBP．1)的表达。结果造模组大鼠血清TG【(1．6±0．69)mmol／L]和TC[(3．14±1．1)mmol／L]含量明显升高。A治疗后，血清T

3、G[(0．69±0．13)mmol／L]和TC[(1．67±1．0)mmol／L]明显降低，肝功能和氧化应激得到了改善，肝脏中的脂质沉积明显减少。SREBP．I的蛋白表达水平明显减低。结论ALA干预可改善高脂饮食联合STZ诱导的SD大鼠的肝脏脂质沉积，其机制可能与改善氧化应激及减少SREBP．1的表达有关。【关键词】硫辛酸；糖尿病，2型；肝脏脂质沉积a-lipoicacidrelievedhepaticsteatosisbyinhibitedHpogenesisindiabetesmellitusratSHIZhi-zhou,G

4、UOZhong-qiu,ⅣYa=qin,WANXin-hong,WUGe,CHENXiao—bing,HUANGJi-ping,CHENYu—hua．DepartmentofEndocrinologyandMetabolism，LonggangDistrictPeopleHospitalShenzhen518172,ChinaCorrespondingauthor：CHENYu-hua,Email．"pangcyh@126．com[Abstract]0bjectiveInthisstudy，weaddressedtheissue

5、sbyexaminingtheefectsofa-lipoicacid(ALA)treatmentforhepaticsteatosisinSDratfedbyHigh-FatcombinedtllSTZ．MethodsMale8weeksSDratwerefromAnimalcentre，GuangdongMedicalCollege．Allratswererandomlydividedintotwogroups：SD+chowdietgroupandSD+HighFatgroupfor8weeks．Afterthat，ino

6、rdertogetthediabetesmodelrats，thehi曲fatgroupratwereinjectedwithSTZ(30mg／kg)．Thenallratswererandomlydividedintotwogroups：DM+salinegroup．DM+ALAgroupfor8weeks．Systemicevaluationswerefollowedwith：BodyweightandFBGweremeasuredevery2weeks．Triglycefides(TG)，totalserumcholest

7、erol(TC)weremeasured．Hematoxylin—eosinstaining(I-IE、andOil—red—Ostainingforliver。ProteinexpressionofSterolregulatoryelementbindingprotein—I(SREBP一1、wastestedbyWestemBlot．ResultsTheresultsshowedALAstatisticallydecreasedthebodyweight，(370．2±30．5)gvs．(509．64-47．8)g，TG(0

8、．694-0．13)mmol／Lvs．(1．64-0．69)mmol／L，TC(1．67±1．03)mmol／Lvs．(14±1．1)mmol／Landoxidativestress．Significantreductionofhepaticli[Iidaccu