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1、华中科技大学硕士学位论文结论：n-3-PUFAs能够降低血清TG、轻微降低血糖，但会导致体重、腹腔脂肪增加及肝脏脂质沉积，其机理与n-3-PUFAs不能抑制SREBP-1c及其下游脂肪合成相关酶FAS及ACC的表达有关。故临床上2型糖尿病患者在应用n-3-PUFAs时需慎重。格列喹酮对肝脏脂质沉积有改善作用。【关键词】n-3多不饱和脂肪酸；2型糖尿病；脂质沉积；SREBP-1c；5华中科技大学硕士学位论文TheInfluenceofn-3PolyunsaturatedFattyAcidsonLipidomicsofLiverinType2DiabeticR
2、atsCandidate：LuMinSupervisor：Prof.WenXiuyingAbstractObjective：Toinvestigatetheeffectofn-3polyunsaturatedfattyacidsonglucoseandlipidmetabolismandlipidomicsofliverintype2diabeticrats.Tostudyitsmolecularmechanismpossibly.Methods：50Wistarratsweredividedrandomlyintonormalcontrolgroup10
3、andmodel40afteroneweeksadaptivefeeding.Buildingtype2diabetesratsmodelwithhigh-fatandhigh-sugardietaddinjectionofstreptozotocin,thecontrolgroupwasinjectedbuffer.The28successfulmodelratsweredividedrandomlyintomodelgroup8,n-3-PUFAsgroupandgliquidonegroup10each.n-3-PUFAsgroupweregiven
4、n-3-PUFAs13.5g/kg/d;gliquidonegroupweregivengliquidoneaqueoussolution135mg/kg/d;modelgroupandnormalgroupweregivendistilledwatercontour;continuedtreatmentfor12weeks.Inthebeginningbeforeadministration,6weeksand12weeksafteradministration,weobservedthebodyweight(BW),serumtriglyceride(
5、TG),totalcholesterol(TC),fastingbloodglucose(FBG).Aftertheendoftheexperiment,weobservedtheitemofintra-abdominalfat,liverwetweight,andcalculatetheindexofintra-abdominalfatandliverindex,andtheliverchangesinthestructure;DetectedTGandTCcontentoflivertissues.RT-PCRdetectionthelivertiss
6、ueofSREBP-1c(SREBP-1c),fattyacidsynthase(FAS)andacetylcoenzymeAcarboxylase(ACC)geneexpression,Western-blotdetectionoflivertissueofSREBP-1cproteinexpression.Results：(1)Thechangeofbodyweightandbodyfat:After6weeksofadministration,gliquidonegroup’sweightwerereducedsignificantly,compar
7、edwiththenormalgroup(P<0.01).After12weeksofadministration,modelandgliquidonegr6华中科技大学硕士学位论文oup’sweightwereloss,comparedwiththenormalgroup(P<0.01orP<0.05);n-3-PUFAsgroup’sweightweregain(P<0.05).n-3-PUFAsgroup’sweightwereincreasedsignificantly，comparedwiththemodelgroup(P<0.01).n-3-P
8、UFAsgroup’sbodyfatwereincreased,c
