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时间:2020-05-07
《乙型肝炎病毒BCP、PreC和C区基因突变与母婴传播免疫失败的相关性-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第34卷第6期中山大学学报(医学科学版)Vo1.34No.62013年11月JOURNALOFSUNYAT-SENUNIVERSITY(MEDICALSCIENCES)Nov.2013乙型肝炎病毒BCP、PreC和C区基因突变与母婴传播免疫失败的相关性尹玉竹,张培珍,章钧,侯红瑛(中山大学附属第三医院产科,广东广州510630)摘要:【目的】探讨BCP、PreC和C区基因突变率及突变热点与HBV母婴传播免疫失败的关系。【方法】选取血清HBsAg阳性且HBVDNA定量>1000U/mL的孕妇,以所分娩的新生儿是否发生免疫失败和孕妇血清HBVDNA定量水平将孕妇分为免疫失败组(19
2、例)和对照1组(免疫成功且HBV—DNA定量≥1.0x107U/mL,48例)、对照2组(免疫成功且HBV-DNA定量<1.0×107U/mL,38例)。对3组孕妇血中HBV进行BCP/PreC/C区基因PCR扩增和测序,比较3组的突变率和突变位点的不同。【结果】免疫失败组与对照l组问HBVDNA定量相比,差异无统计学意义(P>0.05)。但均大于对照2组(P<0.05);BCP、PreC、C区基因突变率,免疫失败组与对照1组问相比,差异无统计学意义(P>0.05),但均低于对照2组(P3、异无统计学意义(P>0.05)。【结论】BCP/PreC/C区基因突变率与HBVDNA定量有关,但未见其与HBV母婴传播免疫失败有明显相关性;也未见tAI762T、ntG1764A、ntG1896A等突变热点与免疫失败的相关性关键词:乙型肝炎病毒:基因突变;母婴传播;免疫失败中图分类号:R714.7文献标志码:A文章编号:1672—3554(2013)06—0873—04RelationshipbetweenBCP,PreC/CGeneMutationandImmunoprophylaxisFailuretoPreventtheVerticalTransmissionofHep4、atitisBvirusYINYu—zhu,ZHANGPei—zhen,ZHANGJun,HOUHong—ying(DepartmentofObstetricsandGynecology,ThirdAflihatedHospital,SUNYat—senUnivemity,Guangzhon510630,China)Abstract:【Objective】Toexploretherelationshipbetweengenemutationfrequency,mutationhotspotsinBCP,PreC/Cgeneandtheimmunoprophylaxisfailu5、retopreventhepatitisBvirus(HBV)verticaltransmission.【Methods】HBsAg-positiveandHBVDNA≥1000U/mLpregnantwomenweredividedintoimmunefailuregroup(19cases)andcontrolgroup1immunesucce,3sfulandmaternal~l'llmHBVDNA≥1.0xl07U/mL,48eases),controlgroup2(imunesuccesfulandmaternal~l'mnHBVDNA<1.0xlo7U/mL.38c6、ases)accordingtotheirneonateswhetheroccurimmunopmphylaxisfailureandmaternal~rlmHBVDNAleve1.MutationfrequencyandmutationalhotspotinBCP/PreC/CgeneweredetectedbyPeRamplificationtechniqueinthreegroups.【Results】TocomparethequantityofHBVDNAamongthethreegroups,thediferenceWilLSnotstatisticallysigni7、ficantbetweenimmunefailuregroupandcontrolgroup1(P>0.05),butallwerehigherthanthecontrolgroup2(P<0.05).TocomparethemutationfrequencyofBCP/PreS/Sgeneamongthe~n'eegroups,thediferencewasnotstatisticallysignificantbetweenimmunefailuregroupandcontrolgroup
3、异无统计学意义(P>0.05)。【结论】BCP/PreC/C区基因突变率与HBVDNA定量有关,但未见其与HBV母婴传播免疫失败有明显相关性;也未见tAI762T、ntG1764A、ntG1896A等突变热点与免疫失败的相关性关键词:乙型肝炎病毒:基因突变;母婴传播;免疫失败中图分类号:R714.7文献标志码:A文章编号:1672—3554(2013)06—0873—04RelationshipbetweenBCP,PreC/CGeneMutationandImmunoprophylaxisFailuretoPreventtheVerticalTransmissionofHep
4、atitisBvirusYINYu—zhu,ZHANGPei—zhen,ZHANGJun,HOUHong—ying(DepartmentofObstetricsandGynecology,ThirdAflihatedHospital,SUNYat—senUnivemity,Guangzhon510630,China)Abstract:【Objective】Toexploretherelationshipbetweengenemutationfrequency,mutationhotspotsinBCP,PreC/Cgeneandtheimmunoprophylaxisfailu
5、retopreventhepatitisBvirus(HBV)verticaltransmission.【Methods】HBsAg-positiveandHBVDNA≥1000U/mLpregnantwomenweredividedintoimmunefailuregroup(19cases)andcontrolgroup1immunesucce,3sfulandmaternal~l'llmHBVDNA≥1.0xl07U/mL,48eases),controlgroup2(imunesuccesfulandmaternal~l'mnHBVDNA<1.0xlo7U/mL.38c
6、ases)accordingtotheirneonateswhetheroccurimmunopmphylaxisfailureandmaternal~rlmHBVDNAleve1.MutationfrequencyandmutationalhotspotinBCP/PreC/CgeneweredetectedbyPeRamplificationtechniqueinthreegroups.【Results】TocomparethequantityofHBVDNAamongthethreegroups,thediferenceWilLSnotstatisticallysigni
7、ficantbetweenimmunefailuregroupandcontrolgroup1(P>0.05),butallwerehigherthanthecontrolgroup2(P<0.05).TocomparethemutationfrequencyofBCP/PreS/Sgeneamongthe~n'eegroups,thediferencewasnotstatisticallysignificantbetweenimmunefailuregroupandcontrolgroup
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