欢迎来到天天文库
浏览记录
ID:5488324
大小:287.00 KB
页数:12页
时间:2017-12-13
《exp.7 糖代谢实验》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、Themeasurementofthebloodsugarandeffectsofadrenalinonbloodsugarconcentration血糖测定及肾上腺素对血糖浓度的影响Exp.7AIM:1、Studytheprincipleandthemethodsofthemeasurementofbloodsugar了解血糖测定的原理和方法;2、Masterthetheoryofinfluenceofadrenalinonbloodsugarconcentration掌握肾上腺素对血糖浓度的影响机制[原理]1、血糖的来源与去路消化吸收糖原分解糖异生氧化供能合成糖原转变为脂肪或氨基酸
2、转变为其他糖糖尿血糖Principle2、Regulationofbloodsugar3、reaction(反应):+2H2O(1)Cu2++COONaCHOHCHOHCOOKCOONaCHOCHOCOOKCuOH-COONaCHOCHOCOOKCu+2H2O+CHO(CHOH)4CH2OHCOOH(CHOH)4CH2OH+Cu2Ored(2)3Cu2O+H3PO4•12MoO3H3PO4•6MoO3•3Mo2O3+6CuOblue420nmOperation(操作)1、Preparationofthebloodfiltraterwithoutprotein?Keepthefilter
3、paperfromwet!!dropping7mlH2O+1mlblood(+oxalate)washadequately,mixscrape,releaseslowly+1/3MH2SO4(1ml)filter+10%Na3WO4(1ml)10minandmixingdroppingandmixing制备两份血滤液:(正常,加肾上腺素)Reagent(ml)StandardglucosesolutionfiltraterH2OCu2+(OH-)solution磷钼酸试剂AddH2OtoS2.00--------2.002.0025.0U1U2--------2.002.00-----
4、---2.002.002.002.0025.025.0O--------2.002.002.0025.0Mixup,100℃for8min,coldwaterfor3min,noshakingMixup,remainfor2min(releaseCO2)2、Themeasurementofbloodsugar1、无蛋白血滤液的制备(两份):①在一侧缓慢放血;②H2SO4、钨酸钠逐滴加入,边加边摇;③滤纸不打湿2、血糖测定①冷水浴冷却3min,勿摇动②颠倒混匀,420nm处比色Attentions(注意事项):3、爱护比色杯!!!Results(结果):mg%=100×ODuODsNO.
5、“0”U1U2SOD420G(mg%)discussionquestions1-4(P28)TSGZ0004-2007(1)基本格式统一封面、目录、鉴定评审结论、明细表(2)有关填写要求见附件G4343谢谢大家
此文档下载收益归作者所有